{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:37:53Z","timestamp":1759963073376},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,1,4]],"date-time":"2016-01-04T00:00:00Z","timestamp":1451865600000},"content-version":"vor","delay-in-days":13548,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Pharma and Therapeutics"],"published-print":{"date-parts":[[1978,12]]},"abstract":"<jats:p>The concentration of diazepam in the plasma and saliva of 9 normal human subjects receiving a single oral dose of diazepam (10 mg) over an 8\u2010hr period was determined by gas\u2010liquid chromatography\/electron capture analysis. In addition, the binding of diazepam to plasma protein in these subjects was determined using equilibrium dialysis at specific time intervals corresponding to periods of plasma and saliva collection. A linear relationship was found between diazepam concentration in plasma and that in both mixed and parotid saliva, over plasma concentrations ranging from 196 \u00b1 16.4 to 74.8 \u00b1 10.3 ng\/ml. The elimination constants (\u2212K<jats:sub>e<\/jats:sub>) were 0.13, 0.17, 0.18 for diazepam disappearance from plasma, parotid saliva, and mixed saliva, respectively. The mean parotid concentration ratio was 0.035 and the mixed saliva\/plasma diazepam concentration ratio was 0.029. These were not affected by variations in plasma diazepam levels. The percentage of the plasma diazepam concentration in both parotid saliva (3.5%) and mixed saliva (2.9%) was of the same order as the fraction of diazepam found to be free from plasma protein in these subjects (2.0% to 3.5%). The results indicate that there is no significant difference between parotid saliva and mixed saliva concentrations over a period of 8 hr after a single oral dose of diazepam. The results strongly suggest that the appearance of diazepam in saliva may provide an alternate, non invasive method of determining plasma diazepam levels.<\/jats:p>","DOI":"10.1002\/cpt1978246720","type":"journal-article","created":{"date-parts":[[2016,1,5]],"date-time":"2016-01-05T02:05:54Z","timestamp":1451959554000},"page":"720-725","source":"Crossref","is-referenced-by-count":37,"title":["Diazepam concentrations in parotid saliva, mixed saliva, and plasma"],"prefix":"10.1111","volume":"24","author":[{"given":"G. John","family":"DiGregorio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony J.","family":"Piraino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eileen","family":"Ruch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,4]]},"container-title":["Clinical Pharmacology &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpt1978246720","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpt1978246720","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpt1978246720","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T13:33:17Z","timestamp":1699363997000},"score":1,"resource":{"primary":{"URL":"https:\/\/ascpt.onlinelibrary.wiley.com\/doi\/10.1002\/cpt1978246720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1978,12]]}},"alternative-id":["10.1002\/cpt1978246720"],"URL":"https:\/\/doi.org\/10.1002\/cpt1978246720","archive":["Portico"],"relation":{},"ISSN":["0009-9236","1532-6535"],"issn-type":[{"value":"0009-9236","type":"print"},{"value":"1532-6535","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,12]]}}}