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Whether specific endogenous lysosomal proteins can bind A2E, a pronounced bisretinoid in lipofuscin granules in retinal pigment epithelial cells, and interfere with enzymatic or photoinduced oxidation of such, has not been explored. Herein, using fluorescence and electronic absorption spectroscopy and mass spectrometry, we demonstrate that Saposin\u2005B, a critical protein in the degradation of sulfatides and \u201cflushing\u201d of lipids, can bind A2E, preventing its H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010dependent enzymatic oxidation by horseradish peroxidase and photooxidation by blue light (<jats:italic>\u03bb<\/jats:italic>=450\u2013460\u2005nm).<\/jats:p>","DOI":"10.1002\/cptc.201700039","type":"journal-article","created":{"date-parts":[[2017,3,23]],"date-time":"2017-03-23T11:25:51Z","timestamp":1490268351000},"page":"256-259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Saposin B Binds the Lipofuscin Bisretinoid A2E and Prevents its Enzymatic and Photooxidation"],"prefix":"10.1002","volume":"1","author":[{"given":"Jay","family":"Tinklepaugh","sequence":"first","affiliation":[{"name":"Department of Chemistry Syracuse University  Syracuse NY 13244 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britannia M.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Chemistry State University of New York at Potsdam  Potsdam NY 13676 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Chemistry Syracuse University  Syracuse NY 13244 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey","family":"Moody","sequence":"additional","affiliation":[{"name":"ICHOR Therapeutics 2521 US-11  Lafayette NY 13084 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kris","family":"Grohn","sequence":"additional","affiliation":[{"name":"ICHOR Therapeutics 2521 US-11  Lafayette NY 13084 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadi","family":"Bou\u2010Abdallah","sequence":"additional","affiliation":[{"name":"Department of Chemistry State University of New York at Potsdam  Potsdam NY 13676 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6786-5656","authenticated-orcid":false,"given":"Robert P.","family":"Doyle","sequence":"additional","affiliation":[{"name":"Department of Chemistry Syracuse University  Syracuse NY 13244 USA"},{"name":"Department of Medicine State University of New York, Upstate Medical University  Syracuse NY 13210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,13]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-vision-111815-114407"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2012.03.009"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1600474113"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1524774113"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja107195u"},{"key":"e_1_2_2_6_1","first-page":"1222","volume":"43","author":"Sparrow J. 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