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Aspects of the entire respiratory system serve as potential targets for candidate toxicants, but each candidate toxicant may impact distinct sites due to differential distribution of either the toxicant or the target cells. Within the conducting airways, the composition of resident cell populations and the metabolic capabilities of the cell populations vary greatly. Thus, studies of this region of the lung require unique, site\u2010selective methods to clearly define the toxic response. Without site\u2010specific sampling, as described in this chapter, the experimental limit of detection for toxicant effects in conducting airways is weakened because differences unrelated to treatment, but related to location, may dominate the response. The protocols included here allow assessment of toxicological responses in the tracheobronchial airways and the gas exchange area of the lung, with specific application to laboratory mammals. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cptx.18","type":"journal-article","created":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T15:49:58Z","timestamp":1485964198000},"source":"Crossref","is-referenced-by-count":7,"title":["Preparation of Specific Compartments of the Lungs for Pathologic and Biochemical Analysis of Toxicologic Responses"],"prefix":"10.1002","volume":"71","author":[{"given":"Laura S.","family":"Van Winkle","sequence":"first","affiliation":[{"name":"Department of Anatomy, Physiology, and Cell Biology School of Veterinary Medicine, University of California Davis California"},{"name":"John Muir Institute for the Environment, Center for Health and the Environment University of California Davis California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacklyn S.","family":"Kelty","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology, and Cell Biology School of Veterinary Medicine, University of California Davis California"},{"name":"John Muir Institute for the Environment, Center for Health and the Environment University of California Davis California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles G.","family":"Plopper","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology, and Cell Biology School of Veterinary Medicine, University of California Davis California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.154.3.8810616"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.159.5.9806044"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/S0022-3565(25)10984-1","article-title":"Relationship of cytochrome P\u2010450 activity to Clara cell cytotoxicity: II Comparison of stereoselectivity of naphthalene epoxidation in lung and nasal mucosa of mouse, hamster, rat and rhesus monkey","volume":"261","author":"Buckpitt A.R.","year":"1992","journal-title":"J. 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