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Intestinal explants can be used in such investigations since they contain all the major intestinal cell types. A detailed procedure to isolate intestinal explants from the rat jejunum is described. A protocol for culturing them <jats:italic>in vitro<\/jats:italic> for up to 24 hr is also provided. \u00a9 2019 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cptx.79","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T12:48:04Z","timestamp":1558615684000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Isolating Rat Intestinal Explants for <i>In Vitro<\/i> Cultures"],"prefix":"10.1002","volume":"80","author":[{"given":"Anjaney","family":"Kothari","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering and Sciences Virginia Tech  Blacksburg Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Padmavathy","family":"Rajagopalan","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering and Sciences Virginia Tech  Blacksburg Virginia"},{"name":"Department of Chemical Engineering Virginia Tech  Blacksburg Virginia"},{"name":"ICTAS Center for Systems Biology of Engineered Tissues Virginia Tech  Blacksburg Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2010.02.004"},{"volume-title":"StatPearls","year":"2019","author":"Collins J.T.","key":"e_1_2_7_3_1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00434-12"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14415"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.101.1.219"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00159-12"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623313518113"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature20168"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.303"},{"volume-title":"Gastrointestinal physiology","year":"2007","author":"Johnson L. 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