{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:30:13Z","timestamp":1788481813596,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T00:00:00Z","timestamp":1713744000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Providing anesthesia and analgesia for mouse subjects is a common and critical practice in the laboratory setting. This practice is necessary for performing invasive procedures, achieving prolonged immobility for sensitive imaging modalities (magnetic resonance imaging, for instance), and providing intra\u2010 and post\u2010procedural pain relief. In addition to facilitating the procedures performed by the investigator, the provision of anesthesia and analgesia is crucial for the preservation of animal welfare and for humane treatment of animals used in research. Furthermore, anesthesia and analgesia are important components of animal use protocols reviewed by Institutional Animal Care and Use Committees, requiring careful consideration and planning for the particular animal model. In this article, we provide technical guidance for the investigator, covering the provision of anesthesia by two routes (injectable and inhalant), guidelines for monitoring anesthesia, current techniques for recognition of pain, considerations for administering preventative analgesia, and considerations for post\u2010operative care. \u00a9 2024 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Injectable anesthesia<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Inhalant anesthesia<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Assessing pain<\/jats:p>","DOI":"10.1002\/cpz1.1006","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T10:05:25Z","timestamp":1713780325000},"source":"Crossref","is-referenced-by-count":1,"title":["Mouse Anesthesia and Analgesia"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2735-8478","authenticated-orcid":false,"given":"Kenzie","family":"Schwartz","sequence":"first","affiliation":[{"name":"Department of Comparative Medicine Stanford University School of Medicine  Stanford California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cholawat","family":"Pacharinsak","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine Stanford University School of Medicine  Stanford California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,22]]},"reference":[{"issue":"5","key":"e_1_2_9_2_1","first-page":"443","article-title":"Optimization of intraperitoneal injection anesthesia in mice: Drugs, dosages, adverse effects, and anesthesia depth","volume":"51","author":"Arras M.","year":"2001","journal-title":"Comparative Medicine"},{"issue":"6","key":"e_1_2_9_3_1","first-page":"916","article-title":"Effects of early atipamezole reversal of medetomidine\u2010ketamine anesthesia in mice","volume":"50","author":"Baker N. 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