{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T23:41:14Z","timestamp":1782862874149,"version":"3.54.5"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T00:00:00Z","timestamp":1716336000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The architecture and morphology of the intestinal tissue from mice or other small animals are difficult to preserve for histological and molecular analysis due to the fragile nature of this tissue. The intestinal mucosa consists of villi and crypts lined with epithelial cells. In between the epithelial folds extends the lamina propria, a loose connective tissue that contains blood and lymph vessels, fibroblasts, and immune cells. Underneath the mucosa are two layers of contractile smooth muscle and nerves. The tissue experiences\u00a0significant changes during fixation, which can impair the reliability of histologic analysis. Poor\u2010quality histologic sections are not suitable for quantitative image\u2010based tissue analysis. This article offers a new fixative composed of neutral buffered formalin (NBF) and acetic acid, called FA. This fixative significantly improved the histology of mouse intestinal tissue compared to traditional NBF and enabled precise, reproducible histologic molecular analyses using QuPath software. Algorithmic training of QuPath allows for automated segmentation of intestinal compartments, which can be further interrogated for cellular composition and disease\u2010related changes. \u00a9 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol<\/jats:bold>: Improved preservation of mouse intestinal tissue using a formalin\/acetic acid fixative<\/jats:p><jats:p><jats:bold>Support Protocol<\/jats:bold>: Quantitative tissue analysis using QuPath<\/jats:p>","DOI":"10.1002\/cpz1.1062","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T08:10:41Z","timestamp":1716365441000},"source":"Crossref","is-referenced-by-count":4,"title":["Improved Preservation of Mouse Intestinal Tissue Using a Formalin\/Acetic Acid Fixative and Quantitative Histological Analysis Using QuPath"],"prefix":"10.1002","volume":"4","author":[{"given":"Tahmineh","family":"Kandelouei","sequence":"first","affiliation":[{"name":"Department of Oncological Sciences, Huntsman Cancer Institute University of Utah  Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Huntsman Cancer Institute University of Utah  Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline","family":"Houghton","sequence":"additional","affiliation":[{"name":"Department of Oncological Sciences, Huntsman Cancer Institute University of Utah  Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice","family":"Knudsen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Huntsman Cancer Institute, Institute of Scientific Computing and Imaging University of Utah  Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3383-2044","authenticated-orcid":false,"given":"Bruce A.","family":"Edgar","sequence":"additional","affiliation":[{"name":"Department of Oncological Sciences, Huntsman Cancer Institute University of Utah  Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,22]]},"reference":[{"key":"e_1_2_7_2_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deab075"},{"key":"e_1_2_7_2_2_1","doi-asserted-by":"crossref","unstructured":"Baker J. 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