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However, due to the difficulties of assessing the early events of infection in humans, most studies of human ascariasis have been restricted to the chronic intestinal phase. Therefore, the <jats:italic>Ascaris<\/jats:italic> mouse model has become a fundamental tool for investigating the immunobiology and pathogenesis of the early infection stage referred to as larval ascariasis because of the model's practicality and ability to replicate the natural processes involved. The <jats:italic>Ascaris<\/jats:italic> mouse model has been widely used to explore factors such as infection resistance\/susceptibility, liver inflammation, lung immune\u2010mediated pathology, and co\u2010infections and, notably, as a pivotal element in preclinical vaccine trials. Exploring the immunobiology of larval ascariasis may offer new insights into disease development and provide a substantial understanding of key components that trigger a protective immune response. This article focuses on creating a comprehensive guide for conducting <jats:italic>Ascaris<\/jats:italic> experimental infections in the laboratory as a foundation for future research efforts. \u00a9 2024 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Acquisition and embryonation of <jats:italic>Ascaris suum<\/jats:italic> eggs from adult females<\/jats:p><jats:p><jats:bold>Alternate Protocol<\/jats:bold>: Cleaning and purification of <jats:italic>Ascaris suum<\/jats:italic> from female <jats:italic>A. suum<\/jats:italic> uteri<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Preparation of <jats:italic>Ascaris suum<\/jats:italic> eggs and murine infection<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Measurement of larval burden and <jats:italic>Ascaris<\/jats:italic>\u2010larva\u2010induced pathogenesis<\/jats:p><jats:p><jats:bold>Basic Protocol 4<\/jats:bold>: <jats:italic>In vitro<\/jats:italic> hatching and purification of <jats:italic>Ascaris<\/jats:italic> L3 larvae<\/jats:p><jats:p><jats:bold>Support Protocol<\/jats:bold>: Preparation of crude antigen from <jats:italic>Ascaris<\/jats:italic> infectious stages<\/jats:p><jats:p><jats:bold>Basic Protocol 5<\/jats:bold>: Ultrastructure\u2010expansion microscopy (U\u2010ExM) of <jats:italic>Ascaris suum<\/jats:italic> larval stages<\/jats:p>","DOI":"10.1002\/cpz1.1074","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T18:14:43Z","timestamp":1719339283000},"source":"Crossref","is-referenced-by-count":4,"title":["<i>Ascaris<\/i> Mouse Model Protocols: Advancing Research on Larval Ascariasis Biology"],"prefix":"10.1002","volume":"4","author":[{"given":"Camila","family":"de Almeida Lopes","sequence":"first","affiliation":[{"name":"Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda Maryland"},{"name":"Laboratory of Immunobiology and Parasites Control Department of Parasitology Federal University of Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianbin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cellular and Molecular Biology University of Tennessee  Knoxville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Liffner","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; 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