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Although immune responses are involved in the development and exacerbation of acute liver injury, the cellular and molecular mechanisms are not fully understood. Intravenous administration of the plant lectin concanavalin A (ConA) is widely used as a model of acute liver injury. ConA triggers T cell activation and cytokine production by crosslinking glycoproteins, including the T cell receptor, leading to the infiltration of myeloid cells into the liver and the subsequent amplification of inflammation in the liver. Thus, the pathogenesis of ConA\u2010induced acute liver injury is considered a model of immune\u2010mediated acute liver injury or autoimmune hepatitis in humans. However, the severity of the liver injury and the analyses of immune cells and non\u2010hematopoietic cells in the liver following ConA injection are significantly influenced by the experimental conditions. This article outlines protocols for ConA\u2010induced acute liver injury in mice and evaluation methods for liver injury, immune cells, and non\u2010hematopoietic cells in the liver. \u00a9 2024 Wiley Periodicals LLC.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : Induction of acute liver injury by ConA injection\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 2<\/jats:bold>\n                    : Evaluation of inflammatory cytokines in mouse plasma\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 3<\/jats:bold>\n                    : Preparation of liver sections and histological analysis of liver injury\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 4<\/jats:bold>\n                    : Preparation of liver immune cells\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 5<\/jats:bold>\n                    : Preparation of hepatocytes, endothelial cells, and hepatic stellate cells\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 6<\/jats:bold>\n                    : Flow cytometry of immune and non\u2010hematopoietic liver cells\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 7<\/jats:bold>\n                    : Flow cytometric sorting of endothelial cells and hepatic stellate cells\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 8<\/jats:bold>\n                    : Quantitative reverse transcription polymerase chain reaction\n                  <\/jats:p>","DOI":"10.1002\/cpz1.1117","type":"journal-article","created":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T07:09:47Z","timestamp":1723273787000},"source":"Crossref","is-referenced-by-count":5,"title":["Concanavalin\u2010A\u2010Induced Acute Liver Injury in Mice"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8386-3607","authenticated-orcid":false,"given":"Tsukasa","family":"Nabekura","sequence":"first","affiliation":[{"name":"Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA) University of Tsukuba  Tsukuba Ibaraki Japan"},{"name":"R&amp;D Center for Innovative Drug Discovery University of Tsukuba  Tsukuba Ibaraki Japan"},{"name":"Division of Immune Response Aichi Cancer Center Research Institute  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0717-4797","authenticated-orcid":false,"given":"Soichi","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of Medicine University of Tsukuba  Tsukuba Ibaraki Japan"},{"name":"Doctoral Program in Medical Science, Graduate School of Comprehensive Human Sciences University of Tsukuba  Tsukuba Ibaraki Japan"},{"name":"Department of Advanced Medical Technologies National Cerebral and Vascular Cancer Center Research Institute  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4480-4858","authenticated-orcid":false,"given":"Akira","family":"Shibuya","sequence":"additional","affiliation":[{"name":"Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA) University of Tsukuba  Tsukuba Ibaraki Japan"},{"name":"R&amp;D Center for Innovative Drug Discovery University of Tsukuba  Tsukuba Ibaraki Japan"},{"name":"Department of Immunology, Institute of Medicine University of Tsukuba  Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,10]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2008.08.009"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/HEP.25893"},{"issue":"4","key":"e_1_2_13_4_1","first-page":"573","article-title":"Non specific cytotoxicity by T cells activated with plant mitogens in vitro and the requirement for plant agents during the killing reaction","volume":"15","author":"Asherson G. 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