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It is well known that there is cellular and microenvironmental heterogeneity within a single tumor mass. Thus, current established protocols for propagating tumor cells <jats:italic>in vitro<\/jats:italic> are limiting because resections obtained from conventional singular samples limit the diversity in cell populations and do not accurately model the heterogeneous tumor. Utilization of discarded tissue obtained from cavitron ultrasonic surgical aspirator (CUSA) of the whole tumor mass allows for establishing novel cell lines <jats:italic>in vitro<\/jats:italic> from the entirety of the tumor, thereby creating an accurate representation of the heterogeneous population of cells originally present in the tumor. Furthermore, while others have described protocols for establishing patient tumor lines once tissue has arrived in the research lab, a primer from the operating room (OR) to the research lab has not been described before. This is integral, as basic research scientists need to understand the surgical environment of the OR, including the methods utilized to obtain a patient's tumor resection, in order to more accurately model cancer biology in laboratory. \u00a9 2021 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Establishment of brain tumor cell lines from patient\u2010derived CUSA samples: processing brain tumor sample from the OR to the lab<\/jats:p><jats:p><jats:bold>Support Protocol 1<\/jats:bold>: Sterilization of microsurgical tools in preparation for dissection<\/jats:p><jats:p><jats:bold>Support Protocol 2<\/jats:bold>: Collagen coating of tissue culture flasks<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Selection of tumor cells <jats:italic>in vitro<\/jats:italic><\/jats:p><jats:p><jats:bold>Support Protocol 3<\/jats:bold>: FACS sorting tumor sample to isolate cancer cells from heterogeneous cell population<\/jats:p>","DOI":"10.1002\/cpz1.140","type":"journal-article","created":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T14:00:17Z","timestamp":1624629617000},"source":"Crossref","is-referenced-by-count":6,"title":["Utilization of Discarded Surgical Tissue from Ultrasonic Aspirators to Establish Patient\u2010Derived Metastatic Brain Tumor Cells: A Guide from the Operating Room to the Research Laboratory"],"prefix":"10.1002","volume":"1","author":[{"given":"Vahan","family":"Martirosian","sequence":"first","affiliation":[{"name":"Department of Neurological Surgery Keck School of Medicine of USC  Los Angeles California"},{"name":"USC Brain Tumor Center  Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krutika","family":"Deshpande","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery Keck School of Medicine of USC  Los Angeles 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