{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:45:15Z","timestamp":1753868715542,"version":"3.41.2"},"reference-count":19,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007135","name":"University of Tennessee","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007135","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Campylobacter jejuni<\/jats:italic> is the leading cause of bacterial\u2010derived gastroenteritis worldwide, infecting 96 million individuals annually. During infection, inflammation and tissue pathology occur in the lower gastrointestinal tract, including the recruitment of leukocytes. Neutrophils are the most abundant leukocyte in humans, and recruitment is associated with bacterial infections and the development of various inflammatory disorders, including inflammatory bowel disease. Neutrophils possess three main antibacterial functions: phagocytosis and degradation of microbes, degranulation to release antimicrobial proteins, and extrusion of neutrophil extracellular traps (NETs). Because neutrophils are recruited to the site of <jats:italic>C. jejuni<\/jats:italic> infection and they are associated with damaging inflammation in other diseases, it is imperative to understand the immunopathology that occurs during <jats:italic>C. jejuni<\/jats:italic> infection and thoroughly study the neutrophil response to the pathogen. Detailed protocols for human and ferret neutrophil isolations, neutrophil gentamicin protection assay, neutrophil activation flow cytometry assay, NET induction and quantification, and neutrophil western blot analysis are included in this article. \u00a9 2021 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Isolation of human and ferret neutrophils<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Neutrophil gentamicin protection assay<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Neutrophil activation flow cytometry analyses<\/jats:p><jats:p><jats:bold>Basic Protocol 4<\/jats:bold>: Neutrophil extracellular trap induction and quantification<\/jats:p><jats:p><jats:bold>Basic Protocol 5<\/jats:bold>: Western blot detection of neutrophil\u2010derived antimicrobial proteins<\/jats:p>","DOI":"10.1002\/cpz1.294","type":"journal-article","created":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T14:33:47Z","timestamp":1637591627000},"source":"Crossref","is-referenced-by-count":4,"title":["Characterization of <i>Campylobacter jejuni<\/i>\u2013Neutrophil Interactions"],"prefix":"10.1002","volume":"1","author":[{"given":"Sean M.","family":"Callahan","sequence":"first","affiliation":[{"name":"Department of Microbiology University of Tennessee  Knoxville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor J.","family":"Hancock","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Tennessee  Knoxville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremiah G.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Tennessee  Knoxville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.06.006"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13210"},{"issue":"1","key":"e_1_2_10_4_1","first-page":"85","article-title":"Campylobacter jejuni infection in the ferret: An animal model of human campylobacteriosis","volume":"48","author":"Fox J. 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