{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:29:58Z","timestamp":1791422998498,"version":"4.3.3"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,12,7]],"date-time":"2021-12-07T00:00:00Z","timestamp":1638835200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The larval zebrafish is an increasingly popular host model for the study of Aspergillosis. The visual accessibility, genetic resources, small size, and ease of handling make zebrafish larvae compatible with higher\u2010throughput investigation of fungal virulence and host resistance mechanisms. This article provides the protocols needed to prepare <jats:italic>Aspergillus fumigatus<\/jats:italic> spore inocula and use microinjection to infect the hindbrain ventricle of zebrafish larvae. Furthermore, we include protocols for analyzing host survival, immobilizing larvae for live imaging, and suggestions for image analysis. \u00a9 2021 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Support Protocol 1<\/jats:bold>: Preparing <jats:italic>Aspergillus<\/jats:italic> spores<\/jats:p><jats:p><jats:bold>Support Protocol 2<\/jats:bold>: Dechorionating zebrafish embryos<\/jats:p><jats:p><jats:bold>Support Protocol 3<\/jats:bold>: Generating transparent larvae with 1\u2010phenyl 2\u2010thiourea (PTU)<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Hindbrain microinjection of zebrafish larvae with <jats:italic>Aspergillus<\/jats:italic> spores<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Survival analysis<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Multi\u2010day imaging of infected larvae<\/jats:p><jats:p><jats:bold>Alternate Protocol<\/jats:bold>: Embedding larvae in low\u2010melting\u2010point agarose<\/jats:p>","DOI":"10.1002\/cpz1.317","type":"journal-article","created":{"date-parts":[[2021,12,7]],"date-time":"2021-12-07T16:54:00Z","timestamp":1638896040000},"source":"Crossref","is-referenced-by-count":12,"title":["Guide to the Larval Zebrafish\u2010<i>Aspergillus<\/i> Infection Model"],"prefix":"10.1002","volume":"1","author":[{"given":"Taylor J.","family":"Schoen","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology University of Wisconsin\u2010Madison  Madison Wisconsin"},{"name":"Comparative Biomedical Sciences Graduate Program University of Wisconsin\u2010Madison  Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Huttenlocher","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology University of Wisconsin\u2010Madison  Madison Wisconsin"},{"name":"Department of Pediatrics University of Wisconsin\u2010Madison  Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy P.","family":"Keller","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology University of Wisconsin\u2010Madison  Madison Wisconsin"},{"name":"Department of Bacteriology University of Wisconsin\u2010Madison  Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,7]]},"reference":[{"key":"e_1_2_13_2_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myaa075"},{"key":"e_1_2_13_2_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof5030067"},{"key":"e_1_2_13_2_3_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1112594"},{"key":"e_1_2_13_2_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2016.1323"},{"key":"e_1_2_13_2_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s1012601\u20100053\u20104"},{"key":"e_1_2_13_2_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00227\u201016"},{"key":"e_1_2_13_2_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00080\u201014"},{"key":"e_1_2_13_2_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2017.05.005"},{"key":"e_1_2_13_2_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201049284\u2010w"},{"key":"e_1_2_13_2_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00140\u201018"},{"key":"e_1_2_13_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2020.103470"},{"key":"e_1_2_13_2_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007229"},{"key":"e_1_2_13_2_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.236539"},{"key":"e_1_2_13_2_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2019.103265"},{"key":"e_1_2_13_2_15_1","doi-asserted-by":"publisher","DOI":"10.3791\/61165"},{"key":"e_1_2_13_2_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.015594"},{"key":"e_1_2_13_2_17_1","volume-title":"The Zebrafish book: A guide for the laboratory use of zebrafish (Brachydanio rerio)","author":"Westerfield M.","year":"1993"},{"key":"e_1_2_13_3_2_1","unstructured":"Huemer K. 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