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Application of cantharidin solution to the ear pinna results in formation of a blister with cell influx and induction of inflammatory mediators at the skin site, as well as local swelling of the ear pinna. The model can be used for investigating anti\u2010inflammatory compounds, such as dexamethasone, and for preclinical drug discovery research, especially in areas where neutrophilic inflammation plays a role in disease pathophysiology. The cantharidin blister model is one of very few translational models described in humans, and the mechanism of inflammation induction is comparable in mice and man. In human studies, the cantharidin blister assay has been used to assess the effects of potential new therapies in early\u2010stage clinical studies. \u00a9 2021 Novartis AG.<\/jats:p><jats:p>This article was corrected on 19 July 2022. See the end of the full text for details.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Application of cantharidin to induce ear inflammation<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Assessment of ear edema<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Assessment of inflammatory mediators in ear tissue<\/jats:p><jats:p><jats:bold>Basic Protocol 4<\/jats:bold>: Histological assessment of ear tissue<\/jats:p>","DOI":"10.1002\/cpz1.49","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T15:06:35Z","timestamp":1613660795000},"source":"Crossref","is-referenced-by-count":2,"title":["Cantharidin\u2010Induced Skin Blister as an In Vivo Model of Inflammation"],"prefix":"10.1002","volume":"1","author":[{"given":"Janet","family":"Dawson","sequence":"first","affiliation":[{"name":"Autoimmunity, Transplantation &amp; Inflammation Novartis Institutes for BioMedical Research  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Vogelsanger","sequence":"additional","affiliation":[{"name":"Autoimmunity, Transplantation &amp; Inflammation Novartis Institutes for BioMedical Research  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523\u20101747.ep12492482"},{"issue":"1","key":"e_1_2_9_3_1","first-page":"22","article-title":"Blister beetles: \u201cSpanish fly","volume":"40","author":"Burnett J. 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