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MI can be induced in neonatal mice surgically by coronary artery ligation. In this protocol, neonates are anesthetized using a combination of inhaled isoflurane anesthesia and induced hypothermia, a significant ethical refinement over previous protocols. A lateral thoracotomy is performed, and an 8\u20100 suture is used to ligate an area below the left atrium to induce MI. The ribs and skin are closed using 7\u20100 sutures, and the pup is rapidly rewarmed with a supply of oxygen. Once recovered, the pup is cleaned and rolled in a fecal bath to mask the smell of surgical interference before being returned to the mother. The surgical procedure is expected to take 10 to 15 min per pup, with a further 5 min for recovery. A dedicated assistant for recovery of pups is recommended to streamline the procedure. \u00a9 2021 Wiley Periodicals LLC.<\/jats:p><jats:p>This article was corrected on 19 July 2022. See the end of the full text for details.<\/jats:p>","DOI":"10.1002\/cpz1.66","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T13:35:15Z","timestamp":1614000915000},"source":"Crossref","is-referenced-by-count":10,"title":["A Refined Protocol for Coronary Artery Ligation in the Neonatal Mouse"],"prefix":"10.1002","volume":"1","author":[{"given":"Carla","family":"De Villiers","sequence":"first","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  United Kingdom"},{"name":"British Heart Foundation Oxbridge Centre of Regenerative Medicine University of Oxford  United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R.","family":"Riley","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  United Kingdom"},{"name":"British Heart Foundation Oxbridge Centre of Regenerative Medicine University of Oxford  United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,22]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.88.1.145"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00246\u2010012\u20100417\u20102"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/chd.12117"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.036086"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.307017"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08899"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.021"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401870208"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1200708"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208863110"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI74418"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201014263\u20102"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000757"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.66","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpz1.66","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.66","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T21:44:23Z","timestamp":1692308663000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.66"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/cpz1.66"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.66","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2]]},"article-number":"e66"}}