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The structural and mechanical complexity of the lung has historically undermined its comprehensive characterization, especially compared to other biological organs, such as the heart or bones. This knowledge void is particularly remarkable when considering that pulmonary disease is one of the leading causes of morbidity and mortality across the globe. Establishing the mechanical properties of the lung is central to formulating a baseline understanding of its operation, which can facilitate investigations of diseased states and how the lung will potentially respond to clinical interventions. Here, we present established and widely accepted experimental protocols for pulmonary material quantification, specifying how to extract, prepare, and test each type of lung constituent under planar biaxial tensile loading to investigate the mechanical properties, such as physiological stress\u2013strain profiles, anisotropy, and viscoelasticity. These methods are presented across an array of commonly studied species (murine, rat, and porcine). Additionally, we highlight how such material properties may inform the construction of an inverse finite element model, which is central to implementing predictive computational tools for accurate disease diagnostics and optimized medical treatments. These presented methodologies are aimed at supporting research advancements in the field of pulmonary biomechanics and to help inaugurate future novel studies. \u00a9 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: General procedures in lung biaxial testing<\/jats:p><jats:p><jats:bold>Alternate Protocol 1<\/jats:bold>: Parenchymal\u2010specific preparation and loading procedures<\/jats:p><jats:p><jats:bold>Alternate Protocol 2<\/jats:bold>: Airway\u2010specific preparation and loading procedures<\/jats:p><jats:p><jats:bold>Alternate Protocol 3<\/jats:bold>: Visceral pleura\u2013specific preparation and loading procedures<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Computational analysis<\/jats:p>","DOI":"10.1002\/cpz1.70001","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T15:13:20Z","timestamp":1725635600000},"source":"Crossref","is-referenced-by-count":12,"title":["Lung Mechanics: Material Characterization of Pulmonary Constituents for an Experimentally Informed Computational Pipeline"],"prefix":"10.1002","volume":"4","author":[{"given":"Talyah M.","family":"Nelson","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering University of California  Riverside California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crystal A.","family":"Mariano","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of California  Riverside California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo O.","family":"Ramirez","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of California  Riverside California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arif","family":"Badrou","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of California  Riverside California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrine A. 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