{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T11:24:02Z","timestamp":1772796242926,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>OPA1 is a dynamin\u2010related GTPase that modulates mitochondrial dynamics and cristae integrity. Humans carry eight different isoforms of OPA1 and mice carry five, all of which are expressed as short\u2010 or long\u2010form isoforms. These isoforms contribute to OPA1's ability to control mitochondrial energetics and DNA maintenance. However, western blot isolation of all long and short isoforms of OPA1 can be difficult. To address this issue, we developed an optimized western blot protocol based on improving running time to isolate five different isoforms of OPA1 in mouse cells and tissues. This protocol can be applied to study changes in mitochondrial structure and function. \u00a9 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol<\/jats:bold>\n                    : Western Blot Protocol for Isolating OPA1 Isoforms in Mouse Primary Skeletal Muscle Cells\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70004","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T04:24:51Z","timestamp":1738729491000},"source":"Crossref","is-referenced-by-count":1,"title":["Creating Optimal Western Blot Conditions for OPA1 Isoforms in Skeletal Muscle Cells and Tissue"],"prefix":"10.1002","volume":"5","author":[{"given":"Margaret","family":"Mungai","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology and Biophysics Vanderbilt University  Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amber","family":"Crabtree","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and 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