{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:39:32Z","timestamp":1792204772760,"version":"4.4.0"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>High\u2010quality DNA with sufficient yield is the goal of DNA extraction protocols. We present an optimized, cost\u2010effective method for extracting next\u2010generation sequencing (NGS)\u2010quality genomic DNA from <jats:italic>Bacillus<\/jats:italic> and <jats:italic>Clostridium<\/jats:italic> species using the chloroform\u2010isoamyl approach. The protocol involves two main procedures: cultivation of the bacteria under appropriate conditions, followed by DNA extraction through cell lysis, phase separation, DNA precipitation, and cleanup. This method has been successfully applied to several hundred strains of <jats:italic>Bacillus<\/jats:italic> and <jats:italic>Clostridium<\/jats:italic> species in our laboratory, including <jats:italic>B. cereus<\/jats:italic>, <jats:italic>B. licheniformis<\/jats:italic>, <jats:italic>C. sporogenes<\/jats:italic>, and <jats:italic>C. tyrobutyricum<\/jats:italic>, demonstrating its efficacy and reliability for producing high\u2010quality DNA that meets NGS quality control standards. \u00a9 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Culturing <jats:italic>Bacillus<\/jats:italic> and <jats:italic>Clostridium<\/jats:italic> species<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: DNA extraction \u00a9 2024 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpz1.70027","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T10:34:26Z","timestamp":1728297266000},"source":"Crossref","is-referenced-by-count":6,"title":["High\u2010Quality Genomic DNA Extraction Protocol for <i>Bacillus<\/i> and <i>Clostridium<\/i> Species"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7552-0299","authenticated-orcid":false,"given":"Yohannes Beyene","family":"Mekonnen","sequence":"first","affiliation":[{"name":"Department of Paraclinical Sciences, Faculty of Veterinary Medicine Norwegian University of Life Sciences (NMBU) \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3175-6022","authenticated-orcid":false,"given":"Marina Elisabeth","family":"Aspholm","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Sciences, Faculty of Veterinary Medicine Norwegian University of Life Sciences (NMBU) \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9522-5461","authenticated-orcid":false,"given":"Ephrem Debebe","family":"Zegeye","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Sciences, Faculty of Veterinary Medicine Norwegian University of Life Sciences (NMBU) \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2144\/97231st04"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713\u201045\u20103\u2010441"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1385\/1\u201059259\u2010038\u20101:29"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/BSAM.S46573"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1987.9999"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.13070\/mm.en.3.191"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2016.10.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-94230-4_3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot093385"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.92206"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060520925594"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","unstructured":"Logan N. 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