{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T03:37:25Z","timestamp":1789270645530,"version":"build-2803163510"},"reference-count":12,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T00:00:00Z","timestamp":1729123200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One of the major concerns following cancer treatment is cardiotoxicity. Therefore, it is important to predict potential cardiotoxicity of cancer chemotherapeutics at the preclinical phase. Current models of cardiotoxicity testing involve either cell culture models or rodent models. We developed a simple invertebrate animal model for rapid screening of cardiotoxicity of cancer chemotherapeutics. <jats:italic>Daphnia magna<\/jats:italic> (water flea, a crustacean) has a transparent body and a large myogenic heart that can be easily monitored under a microscope. Using this model, we have previously described comparative cardiotoxicity of several kinase inhibitors that were approved for the treatment of multiple cancers. In this article, we describe the step\u2010wise protocols for evaluating the heart rate and survival of <jats:italic>D. magna<\/jats:italic> with relevant information on troubleshooting. \u00a9 2024 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Culturing and maintenance of <jats:italic>D. magna<\/jats:italic><\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Experimental design for evaluating heart rate of <jats:italic>Daphnia<\/jats:italic><\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Long\u2010term effect on <jats:italic>Daphnia<\/jats:italic> survival upon drug exposure<\/jats:p>","DOI":"10.1002\/cpz1.70035","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T14:04:03Z","timestamp":1729173843000},"source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of Cardiotoxicity of Cancer Chemotherapeutics Using <i>Daphnia magna<\/i> as a Preclinical Model"],"prefix":"10.1002","volume":"4","author":[{"given":"Qudrathulla Khan Quadri","family":"Mohammed","sequence":"first","affiliation":[{"name":"Molecular Medicine and Therapeutics Laboratory, CPMB Osmania University  Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8146-1551","authenticated-orcid":false,"given":"Rama Krishna","family":"Kancha","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Therapeutics Laboratory, CPMB Osmania University  Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,17]]},"reference":[{"key":"e_1_2_7_2_1","article-title":"Ecology, epidemiology, and evolution of parasitism in Daphnia","author":"Ebert D.","year":"2005","journal-title":"National Library of Medicine, National Center for Biotechnology Information"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.1064"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.912329"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2016.10.018"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2024.116944"},{"key":"e_1_2_7_7_1","volume-title":"Test No. 202: Daphnia sp. Acute Immobilisation Test, OECD Guidelines for the Testing of Chemicals, Section 2","author":"OECD","year":"2004"},{"key":"e_1_2_7_8_1","volume-title":"Test No. 211: Daphnia magna Reproduction Test, OECD Guidelines for the Testing of Chemicals, Section 2","author":"OECD","year":"2012"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2018.04.009"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10091587"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.05.482"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.128660"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctarc.2023.100760"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T09:08:23Z","timestamp":1730192903000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.70035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":12,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1002\/cpz1.70035"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.70035","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10]]},"article-number":"e70035"}}