{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:52:35Z","timestamp":1791492755078,"version":"4.3.4"},"reference-count":8,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The detection of chlorophyll is a convenient method for assessing gene editing efficiency when generating mutations in the <jats:italic>phytoene desaturase<\/jats:italic> (<jats:italic>PDS<\/jats:italic>) gene, which is related to chlorophyll biosynthesis. Various instruments and protocols are available for measuring chlorophyll; however, a minimum leaf area is required for precise quantifications. <jats:italic>In vitro<\/jats:italic> plant regeneration often requires several months to produce enough leaf tissue for chlorophyll content analysis. In this study, we optimized an existing chlorophyll quantification method by using a minimal amount of <jats:italic>in vitro<\/jats:italic> leaf tissue (&lt;2.0 mg) and a microvolume of chlorophyll extraction solution (3 \u00b5l) to quantify chlorophyll <jats:italic>a<\/jats:italic>, chlorophyll <jats:italic>b<\/jats:italic>, and total chlorophyll concentration using a UV\u2010Vis spectrophotometer. This method will serve as a basic protocol for evaluating gene editing efficiency by correlating degrees of albinism (pigment content) with changes in the <jats:italic>PDS<\/jats:italic> gene sequence. Published 2025. This article is a U.S. Government work and is in the public domain in the USA.<\/jats:p><jats:p><jats:bold>Basic Protocol<\/jats:bold>: Determination of chlorophyll content in <jats:italic>in<\/jats:italic> <jats:italic>vitro<\/jats:italic> peanut leaves<\/jats:p>","DOI":"10.1002\/cpz1.70150","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T15:44:43Z","timestamp":1747928683000},"source":"Crossref","is-referenced-by-count":2,"title":["Determination of Chlorophyll Concentration in <i>In<\/i><i>Vitro<\/i> Peanut Leaves"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6925-4610","authenticated-orcid":false,"given":"Paola C.","family":"Faustinelli","sequence":"first","affiliation":[{"name":"USDA\u2010ARS National Peanut Research Laboratory  Dawson Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia N.","family":"Massa","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS National Peanut Research Laboratory  Dawson Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00e9stor W.","family":"Soria","sequence":"additional","affiliation":[{"name":"Universidad Cat\u00f3lica de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana","family":"L\u00f3pez Colomba","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Instituto de Fisiolog\u00eda y Recursos Gen\u00e9ticos Vegetales Unidad de Estudios Agropecuarios (INTA\u2010CONICET)  C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola A.","family":"Su\u00e1rez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Instituto de Fisiolog\u00eda y Recursos Gen\u00e9ticos Vegetales Unidad de Estudios Agropecuarios (INTA\u2010CONICET)  C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marshall C.","family":"Lamb","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS National Peanut Research Laboratory  Dawson Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgeb.2024.100380"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.918"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/csc2.20314"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)48036-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1226911"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.35248\/2329\u20106682.21.10.165"},{"key":"e_1_2_5_8_1","unstructured":"Monteoliva M. I. Bustos D. &Luna C.(2019). Abordajes fisiol\u00f3gicos para el estudio del estr\u00e9s abi\u00f3tico en plantas. Disertaciones y protocolos.Ediciones INTA. Buenos Aires Argentina. 87 p. ISBN 978\u2010987\u2010521\u2010986\u20101."},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.13810"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T14:04:44Z","timestamp":1751033084000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.70150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":8,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/cpz1.70150"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.70150","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5]]},"article-number":"e70150"}}