{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T16:26:24Z","timestamp":1773073584253,"version":"3.50.1"},"reference-count":9,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The placenta is a heterogeneous and complex organ with multiple cell types, posing a challenge for the maternal\u2013fetal medicine field to implement single\u2010cell technologies for a deeper characterization of this essential organ. Several protocols use enzymes to digest the tissue and generate single\u2010cell suspensions, but this approach has several shortcomings, including the loss and reduced viability of cells. In this article, we describe a non\u2010enzymatic approach to generate single\u2010cell suspensions from placental tissue with high yield and viability for single\u2010cell RNA sequencing. \u00a9 2025 Wiley Periodicals LLC.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : Preparation of single\u2010cell suspension from freshly collected third trimester human placental sections\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 2<\/jats:bold>\n                    : Visualization and determination of cell viability\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Alternate Protocol<\/jats:bold>\n                    : Counting cells using a hemocytometer\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70237","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:15:22Z","timestamp":1761236122000},"source":"Crossref","is-referenced-by-count":0,"title":["Non\u2010Enzymatic Generation of Placenta Single Cells From Third Trimester Human Placenta"],"prefix":"10.1002","volume":"5","author":[{"given":"Joy U.","family":"Ameloko","sequence":"first","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria Nigeria"},{"name":"Department of Biochemistry Ahmadu Bello University  Zaria Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7553-438X","authenticated-orcid":false,"given":"Idowu A.","family":"Aimola","sequence":"additional","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria 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B.","family":"Salau","sequence":"additional","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria Nigeria"},{"name":"Department of Biochemistry Ahmadu Bello University  Zaria Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeenat B.","family":"Kudan","sequence":"additional","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria Nigeria"},{"name":"Department of Biochemistry Ahmadu Bello University  Zaria Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruben S.","family":"Baba","sequence":"additional","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria Nigeria"},{"name":"Department of Biochemistry Ahmadu Bello University  Zaria Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solomon","family":"Dunason","sequence":"additional","affiliation":[{"name":"Kaduna Infant Development Birth Cohort Project  Kaduna Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Habiba H.","family":"Abubakar","sequence":"additional","affiliation":[{"name":"Kaduna Infant Development Birth Cohort Project  Kaduna Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalen E.","family":"Audu","sequence":"additional","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joy B.","family":"Karma","sequence":"additional","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria Nigeria"},{"name":"Department of Biochemistry Ahmadu Bello University  Zaria Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peculiar N.","family":"Okoro","sequence":"additional","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria Nigeria"},{"name":"Department of Biochemistry Ahmadu Bello University  Zaria Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abduljabar","family":"Musa","sequence":"additional","affiliation":[{"name":"Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University  Zaria Nigeria"},{"name":"Department of Biochemistry Ahmadu Bello University  Zaria Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musa","family":"Kana","sequence":"additional","affiliation":[{"name":"Kaduna Infant Development Birth Cohort Project  Kaduna Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedict","family":"Anchang","sequence":"additional","affiliation":[{"name":"Biostatistics and Computational Biology Branch National Institute of Environmental Health Sciences Durham North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0209648"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.114.121798"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010022\u201000772\u2010w"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11022"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00819\u201020"},{"key":"e_1_2_8_7_1","unstructured":"Pioli P. 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