{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:53:52Z","timestamp":1764939232914,"version":"3.46.0"},"reference-count":62,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This set of protocols was developed to detect\n                    <jats:italic>Ascochyta rabiei<\/jats:italic>\n                    , the fungus responsible for chickpea blight, in seeds. Given the significant impact of this disease and its potential to cause complete crop loss, even at low inoculum levels, optimizing detection protocols is crucial. This article proposes adjustments to key variables of a basic agar plate test\u2014disinfection method, incubation, illumination, and culture medium\u2014to improve microscopic identification (mainly using a stereomicroscope) and enhance differential growth of\n                    <jats:italic>A. rabiei<\/jats:italic>\n                    relative to other seed\u2010borne fungi. Each step was carefully validated. Detection was verified through morphological and molecular confirmation of\n                    <jats:italic>A. rabiei<\/jats:italic>\n                    , as well as interlaboratory repeatability tests. In addition, the first distribution map of\n                    <jats:italic>A. rabiei<\/jats:italic>\n                    in Argentina was generated based on chickpea seed analyses conducted in the past decade and using the protocols outlined in this article. \u00a9 2025 Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : Seed sampling procedure\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 2<\/jats:bold>\n                    : Preparation of culture medium\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Support Protocol<\/jats:bold>\n                    : Sterilization of antibiotic\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 3<\/jats:bold>\n                    : Seed disinfection by washing\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 4<\/jats:bold>\n                    : Seed sowing and incubation\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 5<\/jats:bold>\n                    : Detection of infected seeds\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70244","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T15:24:33Z","timestamp":1763133873000},"source":"Crossref","is-referenced-by-count":0,"title":["Toward Accurate Detection: Protocol Adjustment for\n                    <i>Ascochyta rabiei<\/i>\n                    in Chickpea Seeds"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7080-7764","authenticated-orcid":false,"given":"Silvina","family":"Pastor","sequence":"first","affiliation":[{"name":"Instituto de Patolog\u00eda Vegetal (IPAVE), Centro de investigaciones Agropecuarias (CIAP) 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