{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T13:27:56Z","timestamp":1769779676070,"version":"3.49.0"},"reference-count":6,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>It is crucial that experiments dealing with plant\u2013pathogen interactions are robust and reproducible. For evaluating the disease\u2010resistance ability of a host plant against any pathogen, controlled conditions are a prerequisite that cannot be obtained with soil\u2010based systems. Variation in environmental conditions during challenge inoculation of a pathogen, when performed in fields or a polyhouse, may lead to spurious results. This is of particular importance when dealing with disease resistance in a fruit tree like pomegranate, as determined after years of research efforts. Due to lack of uniformity and environment variability, the possibility of pathogen escapes and false positives is large under field or polyhouse conditions. To address these limitations, we have used tissue\u2010cultured plants and developed an easy, reliable, rapid, and efficient in vitro bioassay for bacterial blight on pomegranate. Owing to its performance under a closed system, this system minimizes the risk of pathogen escapes, reduces environmental variability, and provides a robust high\u2010throughput screening platform for disease resistance and tissue sampling for downstream functional genomics studies. The protocols enable reproducible experiments to study plant response to bacterial blight infection. Stepwise protocols and detailed instructions are provided to carry out the screening experiment in pomegranate or other similar plant species. \u00a9 2025 Wiley Periodicals LLC.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : Preparation of nodal explants and in vitro rooting\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 2<\/jats:bold>\n                    : In vitro blight bioassay\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 3<\/jats:bold>\n                    : Pathogen re\u2010isolation and confirmation\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70269","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T13:09:26Z","timestamp":1764853766000},"source":"Crossref","is-referenced-by-count":0,"title":["Efficient and Rapid Bioassay for\n                    <i>Xanthomonas citri<\/i>\n                    pv\n                    <i>. punicae<\/i>\n                    Blight on Pomegranate"],"prefix":"10.1002","volume":"5","author":[{"given":"Ruchi","family":"Agarrwal","sequence":"first","affiliation":[{"name":"ICAR\u2010National Research Centre on Pomegranate  Solapur Maharashtra India"},{"name":"These authors contributed equally to this work"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P Roopa","family":"Sowjanya","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Research Centre on Pomegranate  Solapur Maharashtra India"},{"name":"These authors contributed equally to this work"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajinkya A.","family":"Mandave","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Research Centre on Pomegranate  Solapur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Manjunatha","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Research Centre on Pomegranate  Solapur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suvendu","family":"Mondal","sequence":"additional","affiliation":[{"name":"Bhabha Atomic Research Centre  Mumbai Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karuppannan Dhinesh","family":"Babu","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Research Centre on Pomegranate  Solapur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajiv A.","family":"Marathe","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Research Centre on Pomegranate  Solapur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/07060661.2017.1402822"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01006"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants9091214"},{"issue":"2","key":"e_1_2_9_5_1","first-page":"181","article-title":"Effect of temperature, relative humidity and pH on mycelial growth and sporulation of Fusarium solani causing root rot of Indian Aloe (Aloe barbadensis MILL.)","volume":"29","author":"Jat M. K.","year":"2013","journal-title":"Journal of Plant Science and Research"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4454\/jpp.v99i1.3825"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-99-5348-6_18"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70269","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpz1.70269","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70269","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T22:14:17Z","timestamp":1769724857000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.70269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":6,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/cpz1.70269"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.70269","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"article-number":"e70269"}}