{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T15:43:25Z","timestamp":1775663005362,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Multiple myeloma is a bone marrow\u2013derived neoplastic proliferation of plasma cells with low levels of circulating tumor cells. Cytogenetic abnormalities are present in &gt;90% of patients, when assessed by fluorescence in situ hybridization (FISH) on bone marrow, and the specific abnormalities provide vital prognostic information. Provided is a protocol for assessing the high\u2010risk abnormalities del(17p) and amp(1q21) in the circulating plasma cells of myeloma patients by flow cytometry. This utilizes positive plasma cell identification by standard immunophenotyping and then chromosomal analysis by FISH using an imaging flow cytometer. Integrating cell phenotype and FISH in one test, a method called \u201cimmuno\u2010flowFISH\u201d allows for the detection of cytogenetic abnormalities in plasma cells identified by their antigenic profile. This method can be applied to both bone marrow and blood samples to detect primary abnormalities [i.e., hyperdiploidy; immunoglobulin heavy locus (\n                    <jats:italic>IGH<\/jats:italic>\n                    ) translocation] and secondary abnormalities [i.e., del(17p) found in 10% of patients, and gain(1q) present in \u223c40% of patients with myeloma]. Here we describe the protocol for the simultaneous detection of these secondary abnormalities in blood and bone marrow samples from myeloma patients that enables detection of single or \u201cdouble hit\u201d abnormalities, with the latter classified as ultra high\u2013risk disease. The protocol includes the data analysis strategy, as well as the statistics used to confirm the presence of these abnormalities. Applying this method will facilitate blood\u2010based monitoring for the presence and evolution of these critical cytogenetic abnormalities. \u00a9 2026 Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol<\/jats:bold>\n                    : Immuno\u2010flowFISH assessment of del(17p) and amp(1q21) in multiple myeloma\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Support Protocol<\/jats:bold>\n                    : Validation of single fluorophore for positive markers in multiple myeloma\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70305","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T12:24:49Z","timestamp":1769775889000},"source":"Crossref","is-referenced-by-count":0,"title":["Imaging Flow Cytometric Assessment of del(17p) and amp(1q21) in Circulating Plasma Cells of Myeloma Patients"],"prefix":"10.1002","volume":"6","author":[{"given":"Thomas I.","family":"Mincherton","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Harms","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Australia"},{"name":"Medical School The University of Western Australia  Crawley Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah E.","family":"Clarke","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Australia"},{"name":"Haematology Department Fiona Stanley Hospital  Murdoch Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry Y. L.","family":"Hui","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy N.","family":"Erber","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Australia"},{"name":"PathWest Laboratory Medicine  Nedlands Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3462-7501","authenticated-orcid":false,"given":"Kathy A.","family":"Fuller","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"e_1_2_8_2_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010019\u20100519\u20104"},{"key":"e_1_2_8_2_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.08.110192.002053"},{"key":"e_1_2_8_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2024.101168"},{"key":"e_1_2_8_2_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2022.2052658"},{"key":"e_1_2_8_2_5_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.158303"},{"key":"e_1_2_8_2_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp\u20102022\u2010208230"},{"key":"e_1_2_8_2_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.22.01226"},{"key":"e_1_2_8_2_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102141.1998.00623.x"},{"key":"e_1_2_8_2_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.14248"},{"key":"e_1_2_8_2_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23776"},{"key":"e_1_2_8_2_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.26590"},{"key":"e_1_2_8_2_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408\u2010018\u20100153\u20109"},{"key":"e_1_2_8_2_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21961"},{"key":"e_1_2_8_2_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02134"},{"key":"e_1_2_8_2_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"},{"key":"e_1_2_8_3_2_1","unstructured":"Mincherton et\u00a0al. (2024). See above."},{"key":"e_1_2_8_3_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s10038\u2010023\u201001155\u2010z"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70305","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpz1.70305","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T14:43:53Z","timestamp":1775659433000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.70305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,30]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/cpz1.70305"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.70305","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,30]]},"article-number":"e70305"}}