{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T16:05:18Z","timestamp":1777565118221,"version":"3.51.4"},"reference-count":8,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["P01HL152951"],"award-info":[{"award-number":["P01HL152951"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Elevated arterial pressure is a key contributor to cardiovascular disease, yet experimental models that isolate the effects of pressure from confounding systemic factors remain limited. We describe a reproducible surgical protocol to induce mid\u2010thoracic aortic coarctation in mice, generating a stable and quantifiable arterial pressure gradient within the same animal. Using a biocompatible rubber O\u2010ring, a partial stenosis is applied to the descending thoracic aorta of anesthetized C57BL\/6J mice via thoracotomy. The resulting model establishes upstream hypertension while preserving distal perfusion, enabling the investigation of pressure\u2010specific effects on vascular structure and perivascular adipose tissue function. Hemodynamic assessment by radio telemetry and high\u2010frequency Doppler ultrasound confirms significant and sustained gradients in mean and systolic blood pressure across the coarctation site. This model provides a valuable tool for studying pressure\u2010induced remodeling and vascular biology in a controlled and physiologically relevant context. \u00a9 2026 The Author(s).\n                    <jats:italic>Current Protocols<\/jats:italic>\n                    published by Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : Induction of mid\u2010thoracic aortic coarctation using a rubber O\u2010ring\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 2<\/jats:bold>\n                    : Measurement of arterial pressure gradient using two PA\u2010C10 radio telemeters\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 3<\/jats:bold>\n                    : Measurement of blood flow velocity in the descending thoracic aorta using doppler ultrasound\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70318","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:51:03Z","timestamp":1771239063000},"source":"Crossref","is-referenced-by-count":0,"title":["Surgical Induction of Mid\u2010Thoracic Aortic Coarctation in Mice: A Reproducible Preclinical Model of Pressure\u2010Induced Vascular Remodeling"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2752-1124","authenticated-orcid":false,"given":"D. Adam","family":"Lauver","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Garver","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Kreiger\u2010Burke","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1439-777X","authenticated-orcid":false,"given":"C. Javier","family":"Rend\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Andres","family":"Contreras","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie W.","family":"Watts","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory D.","family":"Fink","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI72274"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00332.2020"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.318082"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYP.0000000000000090"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201004096\u20109"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933\u2010024\u201002549\u20109"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00031.2016"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201058368\u2010x"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70318","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpz1.70318","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70318","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T18:45:43Z","timestamp":1777401943000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.70318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":8,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/cpz1.70318"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.70318","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"article-number":"e70318"}}