{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T15:43:29Z","timestamp":1775663009427,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,2]]},"abstract":"Abstract<\/jats:title>\n Acoustic imaging cytometry integrates high\u2010throughput flow cytometry with high\u2010resolution brightfield imaging, enabling simultaneous quantitative and morphological analysis of cells. This technology employs acoustic focusing to align cells precisely within a fluidic channel, ensuring optimal presentation to an optical system that captures high\u2010quality images at rates up to 6000 events per second. The uncolored, high\u2010contrast images reveal detailed cellular morphology, such as size, shape, and internal structures, complementing traditional flow cytometry data like cell counts and fluorescence intensity. This dual\u2010modality approach is transformative for clinical research, offering a comprehensive view of heterogeneous cell populations in fields such as oncology, hematology, and immunology. \u00a9 2026 Wiley Periodicals LLC.<\/jats:p>\n \n Basic Protocol 1<\/jats:bold>\n : Diluting whole blood for flow cytometry of red blood cells\n <\/jats:p>\n \n Basic Protocol 2<\/jats:bold>\n : Lyse\u2010no\u2010wash whole blood staining\n <\/jats:p>\n \n Basic Protocol 3<\/jats:bold>\n : Stain\u2010fix\u2010lysis for leukocyte immunophenotyping and cell cycle analysis\n <\/jats:p>\n \n Basic Protocol 4<\/jats:bold>\n : Setting up the Attune CytPix imaging cytometer\n <\/jats:p>\n \n Basic Protocol 5<\/jats:bold>\n : Manual focusing on the Attune CytPix imaging cytometer\n <\/jats:p>\n \n Basic Protocol 6<\/jats:bold>\n : Red blood cell morphological assessment\n <\/jats:p>\n \n Basic Protocol 7<\/jats:bold>\n : Analysis of cell\u2010to\u2010cell interactions: Cytotoxic activity and rosetting\n <\/jats:p>\n \n Basic Protocol 8<\/jats:bold>\n : Classical morphology and biomarker analysis in myelodysplastic syndromes, plasma cell disorders, and acute myeloid leukemia\n <\/jats:p>\n \n Basic Protocol 9<\/jats:bold>\n : Cell debris discrimination in cerebrospinal fluid analysis\n <\/jats:p>\n \n Basic Protocol 10<\/jats:bold>\n : Real\u2010time single\u2010cell analysis: Discrimination by morphological and pulse parameters\n <\/jats:p>\n \n Basic Protocol 11<\/jats:bold>\n : DNA content and hierarchical clonal heterogeneity analysis in acute myeloid leukemia\n <\/jats:p>","DOI":"10.1002\/cpz1.70323","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:14:29Z","timestamp":1772990069000},"source":"Crossref","is-referenced-by-count":0,"title":["Acoustic Imaging Cytometry for High\u2010Throughput Cell Analysis"],"prefix":"10.1002","volume":"6","author":[{"given":"Marc","family":"Sorigue","sequence":"first","affiliation":[{"name":"Functional Cytomics Lab, Germans Trias i Pujol Research Institute (IGTP), ICO\u2010Hospital Germans Trias i Pujol Universitat Aut\u00f2noma de Barcelona Badalona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebeca","family":"Jurado","sequence":"additional","affiliation":[{"name":"Functional Cytomics Lab, Germans Trias i Pujol Research Institute (IGTP), ICO\u2010Hospital Germans Trias i Pujol Universitat Aut\u00f2noma de Barcelona Badalona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael D.","family":"Ward","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Petriz","sequence":"additional","affiliation":[{"name":"Functional Cytomics Lab, Germans Trias i Pujol Research Institute (IGTP), ICO\u2010Hospital Germans Trias i Pujol Universitat Aut\u00f2noma de Barcelona Badalona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"e_1_2_16_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.70214"},{"key":"e_1_2_16_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpcy.28"},{"key":"e_1_2_16_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2013.01.011"},{"key":"e_1_2_16_5_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005\u20100219"},{"key":"e_1_2_16_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990050502"},{"key":"e_1_2_16_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142956.cy0629s54"},{"key":"e_1_2_16_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751\u2010553X.2008.01100.x"},{"key":"e_1_2_16_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19990815)38:4<153::AID-CYTO2>3.0.CO;2-E"},{"key":"e_1_2_16_10_1","doi-asserted-by":"publisher","DOI":"10.21105\/joss.00861"},{"key":"e_1_2_16_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2017.12.012"},{"key":"e_1_2_16_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12113285"},{"key":"e_1_2_16_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2021.100883"},{"issue":"3","key":"e_1_2_16_14_1","first-page":"585","article-title":"Multidimensional flow cytometric blood cell differentiation without erythrocyte lysis","volume":"17","author":"Terstappen L. 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