{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T03:02:30Z","timestamp":1789441350940,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The choice of cell source for generating induced pluripotent stem cells (iPSCs) is influenced by several factors, including accessibility, reprogramming efficiency, and the invasiveness of the cell collection procedure. Traditional sources such as skin fibroblasts or peripheral blood mononuclear cells often require invasive sampling, which can pose logistical and ethical challenges, especially in large\u2010scale or retrospective studies. In contrast, many biobanks have established collections of easily accessible lymphoblastoid cell lines (LCLs). These LCL repositories constitute an underutilized yet invaluable resource for iPSC research, owing to their widespread availability and existing genetic and phenotypic annotations. Here, we present a robust and reproducible protocol utilizing Sendai virus vectors to reprogram LCLs into iPSCs. Although LCLs are known to exhibit reduced reprogramming efficiency compared to other somatic cell types, our approach optimizes conditions to improve yield and consistency, providing a reliable pathway for generating high\u2010quality iPSCs from archived samples. This methodology enables the reproducible generation of high\u2010quality iPSCs from archived LCLs, eliminating the need for new biospecimen collection. By providing a detailed and optimized protocol, this work lowers technical barriers and facilitates broader adoption of LCL\u2010derived iPSCs across the research community. Ultimately, it expands access to iPSC technology for large\u2010scale, retrospective, and collaborative studies in human development and disease. \u00a9 2026 The Author(s).\n                    <jats:italic>Current Protocols<\/jats:italic>\n                    published by Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>This article was corrected on 25 March 2026. See the end of the full text for details.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : Reprogramming lymphoblastoid cell lines using Sendai virus\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Support Protocol 1<\/jats:bold>\n                    : Pluripotency confirmation by spontaneous trilineage differentiation with TaqMan\u2122 hPSC Scorecard\u2122 panel analysis.\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70342","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:08:14Z","timestamp":1772996894000},"source":"Crossref","is-referenced-by-count":0,"title":["An Optimized Protocol for the Generation of iPSC Lines from Lymphoblastoid Cell Lines Using the Non\u2010integrative Sendai Virus"],"prefix":"10.1002","volume":"6","author":[{"given":"Laurie","family":"Martineau","sequence":"first","affiliation":[{"name":"Centre LOEX de l'Universit\u00e9 Laval, Centre de recherche du CHU de Qu\u00e9bec\u2010Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) Division of Regenerative Medicine  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Bienjonetti","sequence":"additional","affiliation":[{"name":"Centre LOEX de l'Universit\u00e9 Laval, Centre de recherche du CHU de Qu\u00e9bec\u2010Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) Division of Regenerative Medicine  Canada"},{"name":"Department of Surgery, Faculty of Medicine Laval University  Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Bellenfant","sequence":"additional","affiliation":[{"name":"Centre LOEX de l'Universit\u00e9 Laval, Centre de recherche du CHU de Qu\u00e9bec\u2010Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) Division of Regenerative Medicine  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