{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:46:59Z","timestamp":1782474419135,"version":"3.54.5"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,6]]},"abstract":"Abstract<\/jats:title>\n \n Down syndrome (DS) is the most prevalent form of intellectual disability (ID) globally, with an incidence rate of approximately 1 in 1000 births, affecting over 5 million individuals worldwide. DS is characterized by a genetic profile that predisposes individuals to a range of medical and cognitive conditions, including ID and obesity, which place considerable demands on healthcare systems and families alike. Mouse models carrying DS\u2010related genetic mutations offer valuable tools for investigating the pathophysiological mechanisms underlying DS\u2010associated behavioral and metabolic alterations. These models also allow for an evaluation of the impact of both intrinsic and extrinsic environmental factors, aiding in the development of biomarkers and personalized therapies for individuals with DS. In this article, we establish a detailed and comprehensive phenotyping pipeline designed to incorporate high\u2010resolution assessments of cognitive, metabolic, and behavioral variables. Using advanced phenotyping techniques alongside standardized protocols in DS mouse models, this approach systematically captures the variability of DS\u2010associated traits. Our phenotyping pipeline aims to cover the pathways leading to cognitive dysfunction and metabolic imbalances in DS, paving the way for targeted intervention strategies. \u00a9 2026 The Author(s).\n Current Protocols<\/jats:italic>\n published by Wiley Periodicals LLC.\n <\/jats:p>\n \n Basic Protocol 1<\/jats:bold>\n : Diet challenge (chow diet vs. high\u2010fat diet)\n <\/jats:p>\n \n Basic Protocol 2<\/jats:bold>\n : Fecal or saliva microbiota analysis\n <\/jats:p>\n \n Basic Protocol 3<\/jats:bold>\n : Body composition assessment by quantitative nuclear magnetic resonance\n <\/jats:p>\n \n Basic Protocol 4<\/jats:bold>\n : Indirect calorimetry\n <\/jats:p>\n \n Basic Protocol 5<\/jats:bold>\n : Blood collection and processing\n <\/jats:p>\n \n Basic Protocol 6<\/jats:bold>\n : Oral glucose tolerance test\n <\/jats:p>\n \n Basic Protocol 7<\/jats:bold>\n : Long\u2010term monitoring of social groups\n <\/jats:p>\n \n Alternate Protocol 1<\/jats:bold>\n : Analysis of short\u2010term dyadic interactions between unfamiliar mice of the same genotype\n <\/jats:p>\n \n Alternate Protocol 2<\/jats:bold>\n : Analysis of short\u2010term dyadic interactions of mice from the tested strain with an unfamiliar C57BL\/6J mouse\n <\/jats:p>\n \n Basic Protocol 8<\/jats:bold>\n : Intraperitoneal insulin sensitivity test\n <\/jats:p>\n \n Basic Protocol 9<\/jats:bold>\n : Y\u2010maze spontaneous alternation test\n <\/jats:p>\n \n Alternate Protocol 3<\/jats:bold>\n : Y\u2010maze spontaneous alternation test version 2\n <\/jats:p>\n \n Basic Protocol 10<\/jats:bold>\n : Marble\u2010burying analysis\n <\/jats:p>\n \n Alternate Protocol 4<\/jats:bold>\n : Marble\u2010burying analysis version 2\n <\/jats:p>\n \n Basic Protocol 11<\/jats:bold>\n : Object location memory\/novel object recognition\n <\/jats:p>\n \n Alternate Protocol 5<\/jats:bold>\n : Novel object recognition in the Y\u2010maze apparatus\n <\/jats:p>\n \n Basic Protocol 12<\/jats:bold>\n : Nest\u2010building test\n <\/jats:p>\n \n Basic Protocol 13<\/jats:bold>\n : Sucrose preference analysis\n <\/jats:p>\n \n Basic Protocol 14<\/jats:bold>\n : Histopathology \u00a9 2026 by John Wiley & Sons, Inc.\n <\/jats:p>","DOI":"10.1002\/cpz1.70386","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T11:47:36Z","timestamp":1781696856000},"source":"Crossref","is-referenced-by-count":0,"title":["Standardized Pipeline for Metabolism and Cognition in GO\u2010DS21 Mouse Model: Investigating Down Syndrome Comorbidities"],"prefix":"10.1002","volume":"6","author":[{"given":"Mohammed","family":"Selloum","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, PHEN\u2010ICS, CELPHEDIA, UAR2062, US66, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia da","family":"Silva\u2010Buttkus","sequence":"additional","affiliation":[{"name":"Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Riet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, PHEN\u2010ICS, CELPHEDIA, UAR2062, US66, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalia R. V.","family":"Dragano","sequence":"additional","affiliation":[{"name":"Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Neuherberg Germany"},{"name":"German Center for Diabetes Research (DZD) Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian","family":"Garrett","sequence":"additional","affiliation":[{"name":"Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugues","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, PHEN\u2010ICS, CELPHEDIA, UAR2062, US66, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine M.","family":"H\u00f6lter","sequence":"additional","affiliation":[{"name":"Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Torquet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, PHEN\u2010ICS, CELPHEDIA, UAR2062, US66, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Rathkolb","sequence":"additional","affiliation":[{"name":"Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Neuherberg Germany"},{"name":"German Center for Diabetes Research (DZD) Neuherberg Germany"},{"name":"Institute of Molecular Animal Breeding and Biotechnology, Gene Center Ludwig\u2010Maximilians\u2010Universit\u00e4t M\u00fcnchen Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loic","family":"Lindner","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, PHEN\u2010ICS, CELPHEDIA, UAR2062, US66, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Gailus\u2010Durner","sequence":"additional","affiliation":[{"name":"Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Pavlovic","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, PHEN\u2010ICS, CELPHEDIA, UAR2062, US66, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Chan","sequence":"additional","affiliation":[{"name":"Centre for Endocrinology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry Queen Mary University of London London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Beckers","sequence":"additional","affiliation":[{"name":"Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Neuherberg Germany"},{"name":"German Center for Diabetes Research (DZD) Neuherberg Germany"},{"name":"Chair of Experimental Genetics, TUM School of Life Sciences Technische Universit\u00e4t M\u00fcnchen M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit Petit","family":"Demouli\u00e8re","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, PHEN\u2010ICS, CELPHEDIA, UAR2062, US66, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Ey","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, Intitut de G\u00e9n\u00e9tique, Biologie Mol\u00e9culaire et Cellulaire (IGBMC), UMR7104, U1258, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Sorg","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, INSERM, PHEN\u2010ICS, CELPHEDIA, UAR2062, US66, Illkirch Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin Hrabe 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