{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:55:11Z","timestamp":1785855311280,"version":"3.56.0"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    To avoid failure of endodontic treatment, it is important to properly seal the root canals after chemomechanical preparation in order to avoid microleakage. Typically, sealing involves the use of gutta\u2010percha and sealing cement, the latter of which adheres to the dentin and fills the spaces between the gutta\u2010percha and the dentin walls. Laboratory studies have been performed to detect microleakage of endodontic sealers based on various criteria, including microbiological ones; however, none guaranteed that the leakage occurred through the root canal space and not through other pathways. This article proposes a new laboratory assay to evaluate bacterial microleakage in endodontic sealers, ensuring visualization of the correct inoculum infiltration pathway. Furthermore, we present the main critical parameters and provide suggestions on how to manage them. These protocols allow the detection of bacterial microleakage in bovine teeth filled with endodontic sealer and inoculated with\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    . \u00a9 2026 The Author(s).\n                    <jats:italic>Current Protocols<\/jats:italic>\n                    published by Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : Bovine teeth selection and cleaning\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 2<\/jats:bold>\n                    : Bovine teeth inclusion\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 3<\/jats:bold>\n                    : Tooth standardization\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 4<\/jats:bold>\n                    : Set preparation\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 5<\/jats:bold>\n                    : Inoculum preparation\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 6<\/jats:bold>\n                    : Canal inoculation\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 7<\/jats:bold>\n                    : Set incubation\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70407","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:09:18Z","timestamp":1782918558000},"source":"Crossref","is-referenced-by-count":0,"title":["A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6168-3019","authenticated-orcid":false,"given":"Emannuel Fonseca Thom\u00e9 da Silva","family":"Monteiro","sequence":"first","affiliation":[{"name":"Experimental and Applied Microbiology Laboratory Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF)  Nova Friburgo Rio de Janeiro Brazil"},{"name":"Graduate Program in Dentistry Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8037-9553","authenticated-orcid":false,"given":"Alice Abib Ramos","family":"Fabri","sequence":"additional","affiliation":[{"name":"Experimental and Applied Microbiology Laboratory Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF)  Nova Friburgo Rio de Janeiro Brazil"},{"name":"Graduate Program in Dentistry Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6877-2480","authenticated-orcid":false,"given":"Marcelo Barbosa","family":"Garcia","sequence":"additional","affiliation":[{"name":"Graduate Program in Dentistry Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina Silva","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Graduate Program in Dentistry Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1373-3540","authenticated-orcid":false,"given":"Higor Machado","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Graduate Program in Dentistry Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9578-1895","authenticated-orcid":false,"given":"Ana Clara Miranda Porto","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Graduate Program in Dentistry Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6908-5600","authenticated-orcid":false,"given":"Fabr\u00edcia Nogueira","family":"Klein","sequence":"additional","affiliation":[{"name":"Experimental and Applied Microbiology Laboratory Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF)  Nova Friburgo Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9539-0632","authenticated-orcid":false,"given":"Gabriela Ceccon","family":"Chianca","sequence":"additional","affiliation":[{"name":"Experimental and Applied Microbiology Laboratory Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF)  Nova Friburgo Rio de Janeiro Brazil"},{"name":"Graduate Program in Pathology School of Medicine, UFF  Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3283-6059","authenticated-orcid":false,"given":"Roberta","family":"Barcelos","sequence":"additional","affiliation":[{"name":"Department of Specific Formation Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2759-2216","authenticated-orcid":false,"given":"Helv\u00e9cio Cardoso Corr\u00eaa","family":"P\u00f3voa","sequence":"additional","affiliation":[{"name":"Experimental and Applied Microbiology Laboratory Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF)  Nova Friburgo Rio de Janeiro Brazil"},{"name":"Graduate Program in Dentistry Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"},{"name":"Graduate Program in Applied Microbiology and Parasitology Biomedical Institute, UFF  Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2115-6958","authenticated-orcid":false,"given":"Leonardo Santos","family":"Antunes","sequence":"additional","affiliation":[{"name":"Graduate Program in Dentistry Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"},{"name":"Department of Specific Formation Nova Friburgo Institute of Health, UFF  Nova Friburgo Rio de Janeiro Brazil"},{"name":"Graduate Program in Dentistry School of Dentistry, UFF  Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5339-534X","authenticated-orcid":false,"given":"Natalia Lopes Pontes 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Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sdentj.2023.07.004"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/aej.12159"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.90108"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20103913(98)70234\u20103"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20109657.1987.tb00543.x"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3290\/j.jad.a41636"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2015.01.014"},{"key":"e_1_2_13_9_1","first-page":"30","article-title":"Comprehensive review on recent root canal filling materials and techniques\u2013An update","volume":"1","author":"Deshpande P. 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