{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:28:05Z","timestamp":1787740085854,"version":"build-2784847793"},"reference-count":10,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["140437\/2022\u20103;403844\/2021\u20105"],"award-info":[{"award-number":["140437\/2022\u20103;403844\/2021\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In less than a year, SARS\u2010CoV\u20102 managed to displace\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    (Mtb) as the leading cause of death worldwide due to a single infectious agent. Both pathogens affect the respiratory tract, mainly the lungs. However, the impact that a possible Mtb + SARS\u2010CoV\u20102 co\u2010infection can have on the host response is still unknown. Herein we depict a rigorous system to evaluate the complex interaction between two simultaneous infections in vitro in a lung epithelial cell line (A549). Overall, the process includes eight steps: (1) Mtb culture, (2) cell maintenance, (3) preparation of viral stocks, (4) determination of infectious titers, (5) Mtb and SARS\u2010CoV\u20102 co\u2010infection, (6) determination of intracellular bacterial load, (7) SARS\u2010CoV\u20102 viability test, and (8) optional UV\u2010C validation assay for infectivity reduction. This comprehensive protocol will allow experimentalists to study the pathogenesis of co\u2010infection in vitro and facilitate collaborative work in the literature. \u00a9 2026 The Author(s).\n                    <jats:italic>Current Protocols<\/jats:italic>\n                    published by Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol<\/jats:bold>\n                    : In vitro coinfection model using Mtb and SARS\u2010CoV\u20102 in human lung epithelial cells\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70433","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T15:57:57Z","timestamp":1786550277000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Mycobacterium tuberculosis<\/i>\n                    and SARS\u2010CoV\u20102 Co\u2010Infection: An Efficient Cell Model to Study Lung Host\u2013Pathogen Interactions"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1923-5833","authenticated-orcid":false,"given":"Thays Maria Costa","family":"de Lucena","sequence":"first","affiliation":[{"name":"Department of Genetics, Center of Biosciences, Laboratory of Human Genetics and Molecular Biology Federal University of Pernambuco  Recife Brazil"},{"name":"Molecular Biology Sector, Keizo Asami Institute Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e9bora Elienai de Oliveira","family":"Miranda","sequence":"additional","affiliation":[{"name":"Department of Genetics, Center of Biosciences, Laboratory of Human Genetics and Molecular Biology Federal University of Pernambuco  Recife Brazil"},{"name":"Molecular Biology Sector, Keizo Asami Institute Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Vieira de Barros","family":"Arcoverde","sequence":"additional","affiliation":[{"name":"Department of Genetics, Center of Biosciences, Laboratory of Human Genetics and Molecular Biology Federal University of Pernambuco  Recife Brazil"},{"name":"Molecular Biology Sector, Keizo Asami Institute Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Souza Bezerra","family":"Cavalcanti","sequence":"additional","affiliation":[{"name":"Department of Genetics, Center of Biosciences, Laboratory of Human Genetics and Molecular Biology Federal University of Pernambuco  Recife Brazil"},{"name":"Molecular Biology Sector, Keizo Asami Institute Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willyene Marilia","family":"Dantas","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Experimental Therapy, Aggeu Magalh\u00e3es Institute Oswaldo Cruz Foundation  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindomar Jos\u00e9","family":"Pena","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Experimental Therapy, Aggeu Magalh\u00e3es Institute Oswaldo Cruz Foundation  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle Christiane da Silva","family":"Rabello","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, Aggeu Magalh\u00e3es Institute Oswaldo Cruz Foundation  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaqueline","family":"de Azev\u00eado Silva","sequence":"additional","affiliation":[{"name":"Department of Genetics, Center of Biosciences, Laboratory of Human Genetics and Molecular Biology Federal University of Pernambuco  Recife Brazil"},{"name":"Molecular Biology Sector, Keizo Asami Institute Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.18051\/JBiomedKes.2023.v6.363\u2010372"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2025.102672"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1234785"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.992743"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2023.102411"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214\u2010109X(20)30288\u20106"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.radcr.2022.01.063"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.21.0753"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2021.07.029"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02538\u20102021"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70433","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpz1.70433","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70433","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:16:10Z","timestamp":1787739370000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.70433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":10,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/cpz1.70433"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.70433","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"article-number":"e70433"}}