{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:27:08Z","timestamp":1787740028122,"version":"build-2784847793"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This protocol is part of a two\u2010article series [accompanying article: \u201cA biomedical researcher's guide for analyzing short tandem repeat (STR) genotypes of human cell lines and in vitro tissue samples using three standard authentication algorithms\u201d]. Here, we provide a stepwise procedure for using STR genotyping to determine the authenticity and relatedness of human cell lines and tissue samples used in biomedical research using two different cell line similarity tools (CLASTR of Cellosaurus and a provided Microsoft Excel spreadsheet computer file) together with three commonly used matching algorithms. Evaluation of the three algorithms is described in the accompanying article. This workflow protocol should be used in combination with the accompanying article. The applications of these three algorithms (Tanabe, Masters vs Query, and Masters vs Reference) to STR genotyping data are illustrated in the accompanying article. The different types of results are discussed along with suggestions for evaluating and resolving inconclusive match results. \u00a9 2026 Wiley Periodicals LLC.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol<\/jats:bold>\n                    : Analytical workflow for determining the authenticity of human cell lines and tissue samples by\u00a0short tandem repeat (STR) genotype analysis\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70439","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T14:24:46Z","timestamp":1787581486000},"source":"Crossref","is-referenced-by-count":0,"title":["Analytical Workflow for Determining the Authenticity of Human Cell Lines and Tissue Samples by Short Tandem Repeat (STR) Genotype Analysis"],"prefix":"10.1002","volume":"6","author":[{"given":"Christopher T.","family":"Korch","sequence":"first","affiliation":[{"name":"Divisions of Medical Oncology and of Endocrinology, Metabolism, and Diabetes, Department of Medicine University of Colorado Anschutz Medical Campus  Aurora Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Capes\u2010Davis","sequence":"additional","affiliation":[{"name":"CellBank Australia, Children's Medical Research Institute The University of Sydney  Westmead Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Shen","sequence":"additional","affiliation":[{"name":"China Center for Type Culture Collection (CCTCC), College of Life Sciences Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_5_2_1_1","volume-title":"Assay Guidance Manual","author":"Almeida J. 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As of November 2025 it lists information about >167 000 cell lines and has searchable databases of STR genotypes of human mouse and dog STR genotypes."},{"key":"e_1_2_5_3_8_1","unstructured":"https:\/\/www.cellosaurus.org\/str\u2010search\/"},{"key":"e_1_2_5_3_9_1","unstructured":"CLASTR: The STR genotype search tool for the Cellosaurus collection of STR genotypes."},{"key":"e_1_2_5_3_10_1","unstructured":"https:\/\/iclac.org"},{"key":"e_1_2_5_3_11_1","unstructured":"ICLAC: The website of the International Cell Line Authentication Committee provides a great deal of information and guidelines concerning the authentication of cell lines including the register of misidentified cell lines which is the basis for the identification of misidentified cell lines in the Cellosaurus."}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70439","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpz1.70439","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70439","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:16:06Z","timestamp":1787739366000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.70439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":52,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/cpz1.70439"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.70439","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"article-number":"e70439"}}