{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:26:22Z","timestamp":1787739982351,"version":"build-2784847793"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T00:00:00Z","timestamp":1787356800000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["U01AG057562"],"award-info":[{"award-number":["U01AG057562"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["1K08AG084902"],"award-info":[{"award-number":["1K08AG084902"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017644","name":"Sinai Medical Staff Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017644","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Stem cell therapies have emerged as potential therapeutics of interest for many central nervous system diseases, including stroke, epilepsy, neurodegenerative diseases, and traumatic brain injury. Preclinical models are required to evaluate the migration of transplanted stem cells to various areas of the brain, monitor graft survival to improve immunosuppression regimens, and assess stem cell safety and their efficacy on disease outcomes. Understanding all these aspects of stem cells as therapeutics can help optimize parameters for translation to humans. To facilitate this overarching goal, we report a protocol for human stem cell transplantation into mouse fimbria fornix, the outflow white matter tract of the hippocampus, which facilitates stem cell dissemination to regions distant to the injection site. Although our protocol involves injecting human neural stem cells expressing insulin\u2010like growth factor 1 into an Alzheimer's disease mouse model, it can be altered for application to diverse stem cells and cellular therapeutics, secreting various trophic factors, at multiple dosing schemes, and in different central nervous system targets and disorder models. Our approach is robust and highly reproducible, with demonstrated success in improving memory performance in an Alzheimer's disease mouse model and achieving long\u2010term survival of transplanted cells (up to 32 weeks) in immunocompetent mice, facilitating stem cell studies in the context of neuroimmune crosstalk. Our primary protocol involves administering an anti\u2010CD4\/anti\u2010CD40L monoclonal antibody cocktail to prevent graft rejection, with bioluminescence imaging to track transplanted stem cells\n                    <jats:italic>in vivo<\/jats:italic>\n                    . We also present an alternate immunosuppression protocol that relies on tacrolimus and mycophenolate mofetil, which is less costly, albeit less effective (especially in the longer term), than the CD4\u2010CD40L antibody regimen. \u00a9 2026 The Author(s).\n                    <jats:italic>Current Protocols<\/jats:italic>\n                    published by Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol<\/jats:bold>\n                    : Human stem cell transplantation into mouse hippocampus, antibody\u2010based immunosuppression, and\n                    <jats:italic>in vivo<\/jats:italic>\n                    tracking by bioluminescence imaging\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Alternate Protocol<\/jats:bold>\n                    : Immunosuppression by tacrolimus and mycophenolate mofetil\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70442","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T13:31:30Z","timestamp":1787405490000},"source":"Crossref","is-referenced-by-count":0,"title":["Human Stem Cell Transplantation, Immunosuppression, and Noninvasive\n                    <i>In vivo<\/i>\n                    Cell Tracking in the Mouse Brain"],"prefix":"10.1002","volume":"6","author":[{"given":"Kevin S.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology University of Michigan  Ann Arbor MI USA"},{"name":"NeuroNetwork for Emerging Therapies University of Michigan  Ann Arbor MI USA"},{"name":"Department of Neurosurgery University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle J.","family":"Loi","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan  Ann Arbor MI USA"},{"name":"NeuroNetwork for Emerging Therapies University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa M.","family":"McGinley","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan  Ann Arbor MI USA"},{"name":"NeuroNetwork for Emerging Therapies University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osama N.","family":"Kashlan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana M.","family":"Rigan","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan  Ann Arbor MI USA"},{"name":"NeuroNetwork for Emerging Therapies University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shayna N.","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan  Ann Arbor MI USA"},{"name":"NeuroNetwork for Emerging Therapies University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacquelin F.","family":"Kwentus","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan  Ann Arbor MI USA"},{"name":"NeuroNetwork for Emerging Therapies University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Carter","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan  Ann Arbor MI USA"},{"name":"NeuroNetwork for Emerging Therapies University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masha G.","family":"Savelieff","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of North Dakota  Grand Forks ND USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva L.","family":"Feldman","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan  Ann Arbor MI USA"},{"name":"NeuroNetwork for Emerging Therapies University of Michigan  Ann Arbor MI USA"},{"name":"Department of Neurosurgery University of Michigan  Ann Arbor MI 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