{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:01:24Z","timestamp":1790438484590,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This article describes a standardized method for preparing and transporting acute mouse hippocampal brain slices suitable for high\u2010quality single\u2010cell, CA1, pyramidal cells electrophysiological recordings between laboratories in departments or institutions located at distances up to 3 hr apart. The procedure emphasizes maintaining tissue viability and preserving physiological integrity during transport. Slices are prepared in sucrose\u2010based protective solutions under ice\u2010cold, oxygenated conditions, followed by a controlled rewarming period to restore metabolic activity. For transport, slices are submerged in oxygenated artificial cerebrospinal fluid (aCSF) contained in 250\u2010ml parafilm\u2010sealed Duran bottles, ensuring prolonged viability at near\u2010ambient temperature. The method includes parameters for slice thickness, temperature control, oxygenation duration, and maximum transport time to ensure reproducibility across sites. Validation using patch\u2010clamp recordings confirms that electrophysiological properties, such as resting membrane potential, input resistance, action potential firing, and spontaneous synaptic neurotransmission, remain comparable to those in freshly prepared slices. This protocol enables collaborative research where brain slice preparation and electrophysiological recordings occur in separate laboratories, while preserving the reliability and quality of single\u2010cell functional data. This is particularly useful for laboratories located away from animal housing units. \u00a9 2026 The Author(s).\n                    <jats:italic>Current Protocols<\/jats:italic>\n                    published by Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol<\/jats:bold>\n                    : Acute brain slice preparation available for transport\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70461","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:50:30Z","timestamp":1790077830000},"source":"Crossref","is-referenced-by-count":0,"title":["Preparation of Acute Mouse Hippocampal Brain Sections for Transport to a Distant Electrophysiology Laboratory"],"prefix":"10.1002","volume":"6","author":[{"given":"Sofia Ioanna","family":"Nouka","sequence":"first","affiliation":[{"name":"Reading School of Pharmacy University of Reading  Reading United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loukia","family":"Katsouri","sequence":"additional","affiliation":[{"name":"Sainsbury Wellcome Centre for Neural Circuits and Behaviour University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Rudge","sequence":"additional","affiliation":[{"name":"Reading School of Pharmacy University of Reading  Reading United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"O'Keefe","sequence":"additional","affiliation":[{"name":"Sainsbury Wellcome Centre for Neural Circuits and Behaviour University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Tamagnini","sequence":"additional","affiliation":[{"name":"Reading School of Pharmacy University of Reading  Reading United Kingdom"},{"name":"Centro Studi Biomedici Universit\u00e0 degli Studi 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