{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:01:28Z","timestamp":1790438488768,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,9]]},"abstract":"Abstract<\/jats:title>\n \n Chenopodium<\/jats:italic>\n is one of the most complex genera in the family Amaranthaceae, exhibiting ploidy levels ranging from diploid to hexaploid. The lack of distinct morphological characters and phenotypic plasticity has complicated species identification in this genus. The advent of next\u2010generation sequencing technology has enabled in\u2010depth genomic studies of\n Chenopodium<\/jats:italic>\n species. To date, the chloroplast genomes of six Chenopodium species\u2014\n Chenopodium acuminatum<\/jats:italic>\n ,\n C. ficifolium<\/jats:italic>\n ,\n C. berlandieri<\/jats:italic>\n ,\n C. petiolare<\/jats:italic>\n ,\n C. quinoa<\/jats:italic>\n , and\n C. album<\/jats:italic>\n \u2014have been reported using genomic DNA as templates. On the other hand, high\u2010quality chloroplast DNA (cpDNA) is recommended for effective chloroplast genome sequencing. The present study reports an optimized high\u2010salt extraction method for extracting cpDNA from\n C. album<\/jats:italic>\n . The quality of isolated cpDNA was validated by determining the complete chloroplast genome of\n C. album<\/jats:italic>\n using the Illumina platform with a 150 \u00d7 2\u2010pair\u2010end (PE) library and cpDNA as the template. The chloroplast genome of\n C. album<\/jats:italic>\n is 152,028 base pairs and contains 129 genes. Of these, 84 are protein\u2010coding genes, 8 are rRNA genes, and 37 are tRNA genes. This study lays the foundation for isolating high\u2010quality cpDNA using a simple yet efficient method for extracting cpDNA from\n C. album<\/jats:italic>\n , enabling efficient sequencing of the whole plastid genome for further understanding of the evolution, genetics, and taxonomy of this complex plant. \u00a9 2026 Wiley Periodicals LLC\n <\/jats:p>\n \n Basic Protocol<\/jats:bold>\n : An optimized high\u2010salt extraction protocol for chloroplast DNA isolation from\n Chenopodium album<\/jats:italic>\n (Amaranthaceae), enabling whole plastid genome sequencing.\n <\/jats:p>","DOI":"10.1002\/cpz1.70482","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:20:47Z","timestamp":1790436047000},"source":"Crossref","is-referenced-by-count":0,"title":["Optimized Extraction Protocol of Chloroplast DNA from\n Chenopodium album<\/i>\n (Amaranthaceae) Enabling Whole Plastid Genome Sequencing"],"prefix":"10.1002","volume":"6","author":[{"given":"Rajkumari Jashmi","family":"Devi","sequence":"first","affiliation":[{"name":"Plant Systematics and Conservation Laboratory, BRIC Institute of Bioresources and Sustainable Development Takyelpat, Imphal West Manipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merinashwari","family":"Konjengbam","sequence":"additional","affiliation":[{"name":"Plant Systematics and Conservation Laboratory, BRIC Institute of Bioresources and Sustainable Development Takyelpat, Imphal West Manipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biseshwori","family":"Thongam","sequence":"additional","affiliation":[{"name":"Plant Systematics and Conservation Laboratory, BRIC Institute of Bioresources and Sustainable Development Takyelpat, Imphal West Manipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104\u2010024\u201006705\u2010y"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.02.004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002813"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746\u20104811\u20103\u20104"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01696"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13923"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkaf162"},{"key":"e_1_2_9_10_1","unstructured":"Marcheschi A. K. Maughan J. Maughan P. J. Jarvis D. E. Jaggi K. E. &Jellen E. N.(2024). The genome of huauzontle (Chenopodium berlandieri) a North American relative of quinoa.Library\/Life Sciences Undergraduate Poster Competition 2024.https:\/\/scholarsarchive.byu.edu\/library_studentposters_2024\/14"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1139\/g89\u2010029"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00606\u2010003\u20100108\u20109"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/6.5.283"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0207\u2010149"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031468"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084792"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/23802359.2020.1860716"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00061"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12896\u2010024\u201000843\u20108"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438\u2010005\u20100092\u20106"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70482","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpz1.70482","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.70482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:55:03Z","timestamp":1790438103000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.70482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":19,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/cpz1.70482"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.70482","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"value":"2691-1299","type":"print"},{"value":"2691-1299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"article-number":"e70482"}}