{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:39:24Z","timestamp":1792683564145,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Research Council of Finland","doi-asserted-by":"publisher","award":["317437"],"award-info":[{"award-number":["317437"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002341","name":"Research Council of Finland","doi-asserted-by":"publisher","award":["320090"],"award-info":[{"award-number":["320090"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003125","name":"Suomen Kulttuurirahasto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Articular cartilage is a load\u2010bearing connective tissue whose structural integrity depends on a highly specialized extracellular matrix and the activity of resident chondrocytes. In vivo, chondrocytes are continuously exposed to mechanical stimuli, including fluid\u2010induced shear stress associated with synovial fluid movement, which plays a critical role in regulating cell phenotype and matrix homeostasis. Osteoarthritis (OA) is a degenerative joint disease marked by extracellular matrix breakdown, chondrocyte dysfunction, and inflammation. Despite its high prevalence, effective disease\u2010modifying therapies remain unavailable, in part due to the lack of in vitro models that recapitulate both the biochemical and biomechanical environment of native cartilage. This article describes a pumpless, gravity\u2010driven microfluidic platform for modelling inflammatory OA\u2010like conditions under dynamic flow, based on and expanding upon our previously published work. This system is based on gravity\u2010driven flow generated by a rocker platform, eliminating the need for external pumps and enabling scalable, parallelized operation. Defined microchannel geometries allow spatially resolved exposure of cells to varying shear stress levels within a single device. Human articular chondrocytes are cultured under dynamic conditions and stimulated with interleukin\u20101\u03b2 to induce inflammatory responses. The protocols presented here include preparation and extracellular matrix coating of the microfluidic chips, measurement of flow dynamics under rocker\u2010induced motion, long\u2010term dynamic culture of chondrocytes under gravity\u2010driven flow, and induction of OA\u2010like inflammatory conditions followed by evaluation of chondrocyte responses. The platform supports assessment of cellular responses, including viability, metabolic activity, expression of major extracellular matrix markers (collagen type II and aggrecan), and secretion of inflammatory mediators. This approach provides a reproducible in vitro model that integrates dynamic cell culture and inflammation while offering a scalable alternative to conventional static cultures and externally actuated organ\u2010on\u2010chip systems. The method enables efficient investigation of chondrocyte inflammatory responses and has potential applications in OA disease modelling and drug screening. \u00a9 2026 The Author(s).\n                    <jats:italic>Current Protocols<\/jats:italic>\n                    published by Wiley Periodicals LLC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : Extracellular matrix coating and operation of a gravity\u2010driven microfluidic chip for long\u2010term culture of human articular chondrocytes.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Support Protocol 1<\/jats:bold>\n                    : Measurement of gravity\u2010driven flow rate in microfluidic chips under rocker\u2010induced tilt.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 2<\/jats:bold>\n                    : Seeding and long\u2010term dynamic culture of human articular chondrocytes under gravity\u2010driven flow.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 3<\/jats:bold>\n                    : Induction of osteoarthritis\u2010like inflammatory conditions using IL\u20101\u03b2 under gravity\u2010driven flow and analysis of chondrocyte responses.\n                  <\/jats:p>","DOI":"10.1002\/cpz1.70489","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:47:35Z","timestamp":1792680455000},"source":"Crossref","is-referenced-by-count":0,"title":["A Gravity\u2010driven Microfluidic Platform for High\u2010throughput Culture and Inflammatory Stimulation of Human Articular Chondrocytes"],"prefix":"10.1002","volume":"6","author":[{"given":"Aldeliane M. da","family":"Silva","sequence":"first","affiliation":[{"name":"Microelectronics Research Group University of Oulu  Pentti Kaiteran Katu 1 Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meriem","family":"Lamghari","sequence":"additional","affiliation":[{"name":"i3S \u2010 Instituto de Inova\u00e7\u00e3o e Investiga\u00e7\u00e3o em Sa\u00fade Universidade do Porto  Rua Alfredo Allen, 208 Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoang\u2010Tuan","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Proteins.1  Espoo Finland"},{"name":"Finnadvance Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jere","family":"Kettunen","sequence":"additional","affiliation":[{"name":"Finnadvance Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastien","family":"Mosser","sequence":"additional","affiliation":[{"name":"Finnadvance Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prateek","family":"Singh","sequence":"additional","affiliation":[{"name":"Proteins.1  Espoo Finland"},{"name":"Finnadvance Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Mobasheri","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu  Oulu Finland"},{"name":"Department of Personalized Medicine State Research Institute Centre for Innovative Medicine  Vilnius Lithuania"},{"name":"Department of Joint Surgery First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 de Li\u00e8ge  Li\u00e8ge Belgium"},{"name":"Department for Health Sciences, Medicine and Research, Center for Regenerative Medicine, Faculty of Health and Medicine University for Continuing Education Krems  Krems Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela S.","family":"Lorite","sequence":"additional","affiliation":[{"name":"Microelectronics Research Group University of Oulu  Pentti Kaiteran Katu 1 Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila Campioni","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Microelectronics Research Group University of Oulu  Pentti Kaiteran Katu 1 Oulu 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