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The engraftment of these cells and their development into human lymphocytes has been made possible by the development of novel severely immunodeficient mouse strains. Proven protocols for the generation and analysis of humanized mice in the NSG mouse background are presented here. \u00a9 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Human umbilical stem cell engraftment of neonatal immunodeficient mice<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Human umbilical stem cell engraftment of 4\u2010week\u2010old immunodeficient mice<\/jats:p><jats:p><jats:bold>Support Protocol 1<\/jats:bold>: Preparation of human umbilical stem cells<\/jats:p><jats:p><jats:bold>Support Protocol 2<\/jats:bold>: Submandibular blood collection from humanized mice and analysis of peripheral blood via flow cytometry<\/jats:p>","DOI":"10.1002\/cpz1.800","type":"journal-article","created":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T13:42:43Z","timestamp":1686663763000},"source":"Crossref","is-referenced-by-count":5,"title":["Production of Humanized Mice through Stem Cell Transfer"],"prefix":"10.1002","volume":"3","author":[{"given":"Cameron","family":"Phelps","sequence":"first","affiliation":[{"name":"Department of Veterinary Biosciences The Ohio State University  Columbus Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devra D.","family":"Huey","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences The Ohio State University  Columbus Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Niewiesk","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences The Ohio State University  Columbus Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,13]]},"reference":[{"key":"e_1_2_9_2_1_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044219"},{"key":"e_1_2_9_2_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201008\u2010301507"},{"key":"e_1_2_9_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.12.008"},{"key":"e_1_2_9_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/1074\u20107613(95)90047\u20100"},{"key":"e_1_2_9_2_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2015.0289"},{"key":"e_1_2_9_2_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810378282"},{"key":"e_1_2_9_2_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102004\u201002\u20100656"},{"key":"e_1_2_9_2_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301\u2010472X(99)00078\u20108"},{"key":"e_1_2_9_2_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088205"},{"key":"e_1_2_9_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2015.09.005"},{"issue":"1","key":"e_1_2_9_2_11_1","first-page":"4","article-title":"Role of wild\u2010type and recombinant human T\u2010cell leukemia viruses in lymphoproliferative disease in humanized NSG mice","volume":"68","author":"Huey D. 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