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Data gathered from multiple single\u2010cell\/single\u2010nucleus and spatial molecular assays will be used to build a 3D molecular atlas. Herein, we describe our multistep protocol, beginning with an optimized organ procurement workflow that maintains functional characteristics of the uterus, ovaries, and fallopian tubes by perfusing these organs with preservation solution. We have also developed a structured tissue sampling procedure that retains information on individual\u2010level anatomic, physiologic, and individual diversity of the female reproductive system, toward full exploration of the function and structure of female reproductive cells. \u00a9 2023 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Preparation and preservation of the female reproductive system (ovaries, fallopian tubes, and uterus) prior to procurement<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Removal of the female reproductive system <jats:italic>en bloc<\/jats:italic><\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Postsurgical dissection of ovaries<\/jats:p><jats:p><jats:bold>Basic Protocol 4<\/jats:bold>: Postsurgical dissection of fallopian tubes<\/jats:p><jats:p><jats:bold>Basic Protocol 5<\/jats:bold>: Postsurgical dissection of cervix<\/jats:p><jats:p><jats:bold>Basic Protocol 6<\/jats:bold>: Postsurgical dissection of uterine body<\/jats:p><jats:p><jats:bold>Support Protocol 1<\/jats:bold>: OCT\u2010embedded tissue protocol<\/jats:p><jats:p><jats:bold>Support Protocol 2<\/jats:bold>: Tissue fixation protocol<\/jats:p><jats:p><jats:bold>Support Protocol 3<\/jats:bold>: Snap\u2010frozen tissue protocol<\/jats:p><jats:p><jats:bold>Basic Protocol 7<\/jats:bold>: Tissue slice preparation for Visium analysis<\/jats:p><jats:p><jats:bold>Support Protocol 4<\/jats:bold>: Hematoxylin and eosin staining for 10X Visium imaging<\/jats:p><jats:p><jats:bold>Basic Protocol 8<\/jats:bold>: Manual tissue dissociation for\u00a0Multiome analysis<\/jats:p><jats:p><jats:bold>Basic Protocol 9<\/jats:bold>: Tissue dissociation for\u00a0Multiome analysis using S2 Singulator<\/jats:p>","DOI":"10.1002\/cpz1.848","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T05:51:57Z","timestamp":1692424317000},"source":"Crossref","is-referenced-by-count":1,"title":["Systematic Sampling 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Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise C.","family":"Laurent","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences University of California San Diego  La Jolla California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hieu","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Arts and Sciences University of Pennsylvania  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Renz","sequence":"additional","affiliation":[{"name":"Gift of Life Donor Program  Philadelphia PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean G.","family":"Rosario","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Arts and Sciences University of Pennsylvania  Philadelphia 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