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This protocol describes murine models of acute <jats:italic>T. gondii<\/jats:italic> infection, toxoplasmic encephalitis and toxoplasma retinochoroiditis. <jats:italic>T. gondii<\/jats:italic> infection in severe combined immunodeficient (SCID) mice, deficient in T and B cells, has allowed for the study of T cell\u2010independent mechanisms of defense against intracellular organisms, as described here. The uracil auxotroph strain <jats:italic>cps1\u20101<\/jats:italic> and temperature\u2010sensitive mutant strains of <jats:italic>T. gondii<\/jats:italic> induce protection against challenge with virulent strains of the parasite. They have allowed studies of immunization and adoptive\u2010transfer experiments. A protocol is provided for infection with these mutant strains. The EGS strain of <jats:italic>T. gondii<\/jats:italic> has the unique feature of spontaneously forming tissue cysts in cell culture. Dual fluorescent reporter stains of this strain have allowed the study of tachyzoite to bradyzoite transitions in vitro and in vivo. A protocol for in vitro and in vivo growth of this strain and tissue cyst isolation is provided. Genetic manipulation of <jats:italic>T. gondii<\/jats:italic> and mice has led to the development of parasites that express fluorescent proteins as well as mice with fluorescently labeled leukocytes. This together with the use of <jats:italic>T. gondii<\/jats:italic> that express model antigens and transgenic mice that express the appropriate T cell receptor have facilitated the in vivo study of parasite host\u2010interaction. In addition, parasites that express bioluminescent markers have made it possible to study the dynamics of infection in real time using bioluminescence imaging. Support protocols present methodology for evaluation of progression of infection and immune response to the parasite that includes these newer methodologies. In addition, support protocols address the maintenance of <jats:italic>T. gondii<\/jats:italic> tissue cysts and tachyzoites, as well as preparation of <jats:italic>T. gondii<\/jats:italic> lysate antigens. \u00a9 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Induction of acute <jats:italic>T. gondii<\/jats:italic> infection in mice<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Model of toxoplasmic encephalitis and toxoplasma retinochoroiditis in chronically infected mice<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Assessment of <jats:italic>T. gondii<\/jats:italic> invasion into neural tissue<\/jats:p><jats:p><jats:bold>Basic Protocol 4<\/jats:bold>: <jats:italic>T. gondii<\/jats:italic> infection in <jats:italic>scid\/scid<\/jats:italic> (SCID) mice<\/jats:p><jats:p><jats:bold>Basic Protocol 5<\/jats:bold>: Infection with the uracil auxotroph strain <jats:italic>CPS1\u20101<\/jats:italic> or the temperature\u2010sensitive TS\u20104 strain of <jats:italic>T. gondii<\/jats:italic><\/jats:p><jats:p><jats:bold>Basic Protocol 6<\/jats:bold>: In vivo and in vitro maintenance of the EGS strain of <jats:italic>T. gondii<\/jats:italic><\/jats:p><jats:p><jats:bold>Support Protocol 1<\/jats:bold>: Assessment of progression of infection and immune response to <jats:italic>T. gondii<\/jats:italic><\/jats:p><jats:p><jats:bold>Support Protocol 2<\/jats:bold>: Maintenance of a bank of <jats:italic>T. gondii<\/jats:italic> cysts of the ME49 strain<\/jats:p><jats:p><jats:bold>Support Protocol 3<\/jats:bold>: Maintenance of <jats:italic>T. gondii<\/jats:italic> tachyzoites using human foreskin fibroblasts<\/jats:p><jats:p><jats:bold>Support Protocol 4<\/jats:bold>: Maintenance of <jats:italic>T. gondii<\/jats:italic> tachyzoites in mice<\/jats:p><jats:p><jats:bold>Support Protocol 5<\/jats:bold>: Preparation of <jats:italic>T. gondii<\/jats:italic> lysate antigens<\/jats:p><jats:p><jats:bold>Support Protocol 6<\/jats:bold>: Isolation of <jats:italic>T. gondii<\/jats:italic> tissue cysts from brain<\/jats:p>","DOI":"10.1002\/cpz1.871","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T13:36:58Z","timestamp":1694439418000},"source":"Crossref","is-referenced-by-count":7,"title":["Animal Models for <i>Toxoplasma gondii<\/i> Infection"],"prefix":"10.1002","volume":"3","author":[{"given":"Carlos","family":"S. 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