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The members of these species differ considerably in their genomic characteristics and also in their tropism and pathogenicity. Virus isolation in cell culture remains critical for the preservation and comprehensive characterization of viruses of biomedical interest but has been almost completely abandoned by diagnostic laboratories. Currently, the most frequently used approach for the detection of HAdV in clinical specimens is real\u2010time qPCR targeting a region of the hexon gene, conserved among all genotypes described to the present. In the absence of typing, the detection of an HAdV in association with disease provides limited information. Molecular typing is therefore highly desirable and required in the epidemiological investigation of HAdV\u2010associated disease. \u00a9 2023 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Virus isolation from plasma and whole blood<\/jats:p><jats:p><jats:bold>Alternate Protocol 1<\/jats:bold>: Virus isolation from stool<\/jats:p><jats:p><jats:bold>Alternate Protocol 2<\/jats:bold>: Virus isolation from respiratory specimens and urine<\/jats:p><jats:p><jats:bold>Alternate Protocol 3<\/jats:bold>: Virus isolation from tissue specimens<\/jats:p><jats:p><jats:bold>Support Protocol<\/jats:bold>: Inoculation of shell vials<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Extraction of highly pure viral genomic DNA from infected cells<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Molecular detection of human adenovirus by real\u2010time PCR<\/jats:p><jats:p><jats:bold>Basic Protocol 4<\/jats:bold>: Molecular typing for basic identification of species and hexon type<\/jats:p><jats:p><jats:bold>Basic Protocol 5<\/jats:bold>: Typing human adenoviruses by next\u2010generation whole\u2010genome sequencing and analysis<\/jats:p>","DOI":"10.1002\/cpz1.916","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T15:28:52Z","timestamp":1700148532000},"source":"Crossref","is-referenced-by-count":7,"title":["Characterization of Human Adenoviruses of Medical Importance: Isolation of Infectious Virus from Clinical Specimens and Molecular Typing"],"prefix":"10.1002","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6499-9579","authenticated-orcid":false,"given":"Adriana E.","family":"Kajon","sequence":"first","affiliation":[{"name":"Lovelace Biomedical Research Institute  Albuquerque New Mexico USA"},{"name":"Wadsworth Center, New York State Department of Health David Axelrod Institute  Albany New York 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