{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:41:42Z","timestamp":1762868502976,"version":"3.41.2"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,4,5]],"date-time":"2021-04-05T00:00:00Z","timestamp":1617580800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Protocols"],"published-print":{"date-parts":[[2021,4]]},"abstract":"Abstract<\/jats:title>Pneumocystis jirovecii<\/jats:italic> can cause severe pneumonia in immunocompromised patients, which can be life threatening if left untreated. Despite the widespread use of polymerase chain reaction (PCR) within the clinical laboratory setting, FDA\u2010approved PCR assays are not readily available for the detection of Pneumocystis from respiratory samples. Using the Luminex ARIES system\u2014an open\u2010channel, automated, sample\u2010to\u2010answer PCR platform\u2014the cell division cycle 2 (cdc\u20102<\/jats:italic>) gene can be targeted for the detection of Pneumocystis. This novel TaqMan\u2010based, real\u2010time PCR assay offers improved sensitivity compared to staining or immunofluorescence while reducing turnaround time and eliminating the challenges surrounding microscopic identification. \u00a9 2021 Wiley Periodicals LLC.<\/jats:p>Basic Protocol 1<\/jats:bold>: Primer\/probe master mix preparation<\/jats:p>Basic Protocol 2<\/jats:bold>: Positive control (cdc\u20102<\/jats:italic>) plasmid preparation<\/jats:p>Basic Protocol 3<\/jats:bold>: Mucus digestion<\/jats:p>Basic Protocol 4<\/jats:bold>: Cell lysis<\/jats:p>Basic Protocol 5<\/jats:bold>: Carrier RNA\/proteinase K preparation<\/jats:p>Basic Protocol 6<\/jats:bold>: Cassette assembly<\/jats:p>Basic Protocol 7<\/jats:bold>: Running the assay<\/jats:p>Basic Protocol 8<\/jats:bold>: Interpreting results<\/jats:p>","DOI":"10.1002\/cpz1.95","type":"journal-article","created":{"date-parts":[[2021,4,5]],"date-time":"2021-04-05T13:59:36Z","timestamp":1617631176000},"source":"Crossref","is-referenced-by-count":2,"title":["Detection of Pneumocystis jirovecii<\/i> from Clinical Specimens Utilizing a TaqMan\u2010Based Real\u2010Time PCR Assay on the Luminex ARIES"],"prefix":"10.1002","volume":"1","author":[{"given":"Kyle","family":"Albulushi","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin School of Medicine and Public Health Madison Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brittney","family":"Jung\u2010Hynes","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin School of Medicine and Public Health Madison Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derrick","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin School of Medicine and Public Health Madison Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,5]]},"reference":[{"key":"e_1_2_13_2_1_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074\u201002762009000300004"},{"key":"e_1_2_13_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.08.006"},{"key":"e_1_2_13_2_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw085"},{"key":"e_1_2_13_2_4_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0716\u201010182014000400007"},{"key":"e_1_2_13_2_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.2.490\u2010494.2002"},{"key":"e_1_2_13_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2012.08.024"},{"key":"e_1_2_13_2_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc6806"},{"key":"e_1_2_13_2_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199001183220304"},{"key":"e_1_2_13_2_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010002\u20100758\u20105"},{"key":"e_1_2_13_2_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2009.131668"},{"key":"e_1_2_13_2_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010015\u20104678\u20106"},{"key":"e_1_2_13_2_12_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0809.020096"},{"key":"e_1_2_13_2_13_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.18.3.3122"},{"key":"e_1_2_13_3_2_1","unstructured":"https:\/\/www.cdc.gov\/fungal\/diseases\/pneumocystis\u2010pneumonia"},{"key":"e_1_2_13_3_4_1","unstructured":"https:\/\/www.uwhealth.org\/files\/uwhealth\/docs\/about\/uw_health_facts_and_figures.pdf"}],"container-title":["Current Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.95","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpz1.95","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpz1.95","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T15:26:35Z","timestamp":1693581995000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpz1.95"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1002\/cpz1.95"],"URL":"https:\/\/doi.org\/10.1002\/cpz1.95","archive":["Portico"],"relation":{},"ISSN":["2691-1299","2691-1299"],"issn-type":[{"type":"print","value":"2691-1299"},{"type":"electronic","value":"2691-1299"}],"subject":[],"published":{"date-parts":[[2021,4]]},"article-number":"e95"}}