{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T12:11:02Z","timestamp":1774008662275,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005366","name":"Universitetet i Oslo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp; Exp Dental Res"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The presence of and the causative role of high\u2010risk human papilloma virus (HPV) is a subject of controversy in oral squamous cell carcinoma (OSCC). The disagreement can be related to the misclassification of OSCC as oropharyngeal squamous cell carcinoma and\/or lack of standard detection methods. This study aimed to examine the presence of transcriptionally active high\u2010risk HPV in a homogenous Norwegian cohort of primary and second primary OSCC of the mobile tongue (oral tongue squamous cell carcinoma\u2014OTSCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tissue microarrays containing formalin\u2010fixed and paraffin\u2010embedded cores of 146 OTSCC from the anterior 2\/3 of the tongue (n = 128 primary and n = 18\u2009second primary) from a multicentric Norwegian cohort were examined for the presence of high\u2010risk HPV by DNA\u2010 and RNA\u2010in situ hybridization (ISH) assays and p16 immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Transcriptionally active HPV (<jats:italic>E6\/E7<\/jats:italic> mRNA) was not identified in any of the OTSCC specimens. In parallel, no tumors were positive for HPV by DNA ISH. Although, 61 (42%) OTSCC demonstrated p16 positivity with varying staining intensity and subcellular localization, only two cases demonstrated strong and uniform p16\u2010staining (both cytoplasmic and nuclear) in &gt;70% of cancer cells. The absence of transcriptionally active high\u2010risk HPV in this cohort of OTSCC indicates that high\u2010risk HPV is an unlikely causative factor in the present material.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.342","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T13:10:40Z","timestamp":1604409040000},"page":"70-77","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["High\u2010risk human papilloma virus was not detected in a Norwegian cohort of oral squamous cell carcinoma of the mobile tongue"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1687-3247","authenticated-orcid":false,"given":"Tine M.","family":"S\u00f8land","sequence":"first","affiliation":[{"name":"Faculty of Dentistry, Institute of Oral Biology University of Oslo  Oslo Norway"},{"name":"Department of Pathology, Rikshospitalet Oslo University Hospital  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inger\u2010Heidi","family":"Bjerkli","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology University Hospital of North Norway  Troms\u00f8 Norway"},{"name":"Department of Medical Biology, Faculty of Health Sciences UiT The Arctic University of Norway  Troms\u00f8 Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanette B.","family":"Georgsen","sequence":"additional","affiliation":[{"name":"Department of Pathology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olaf","family":"Schreurs","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Institute of Oral Biology University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Jebsen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rikshospitalet Oslo University Hospital  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helene","family":"Laurvik","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rikshospitalet Oslo University Hospital  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0061-825X","authenticated-orcid":false,"given":"Dipak","family":"Sapkota","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Institute of Oral Biology University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16918-w"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0912217"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318265fb2b"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0227738"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2009.08.018"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780204"},{"key":"e_1_2_8_8_1","unstructured":"Danish\u2010Head\u2010and\u2010Neck\u2010Cancer\u2010Group. (2012).DAHANCA\/EORTC guidelines for scoring and classification of p16 immunohistochemistry in HPV related oropharyngeal cancer Retrieved fromhttps:\/\/www.dahanca.dk\/assets\/files\/GUID_Scoring%20and%20classification%20of%20p16.pdf"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-017-4748-8"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1934"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-6053(02)00094-7"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2017.05.026"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-012-0368-1"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cre2.153"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cjp2.111"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.04169.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.path.2018.04.002"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874210601206010126"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dji237"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.11.011"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-017-0792-3"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70471-1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.24589"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.01.006"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2019.05.006"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23183"},{"issue":"6","key":"e_1_2_8_27_1","first-page":"418","article-title":"Morphological and immunohistochemical evidence suggesting human papillomavirus (HPV) involvement in oral squamous cell carcinogenesis","volume":"12","author":"Syrj\u00e4nen K.","year":"1983","journal-title":"International Journal of Oral and Maxillofacial Surgery"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djv344"},{"issue":"85","key":"e_1_2_8_29_1","first-page":"1","article-title":"RNAscope for in situ detection of transcriptionally active human papillomavirus in head and neck squamous cell carcinoma","volume":"11","author":"Wang H.","year":"2014","journal-title":"J Vis Exp 2014"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.05.004"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/b137945"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000485322"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2019.02.001"}],"container-title":["Clinical and Experimental Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cre2.342","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cre2.342","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cre2.342","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T02:29:47Z","timestamp":1693708187000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cre2.342"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,3]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/cre2.342"],"URL":"https:\/\/doi.org\/10.1002\/cre2.342","archive":["Portico"],"relation":{},"ISSN":["2057-4347","2057-4347"],"issn-type":[{"value":"2057-4347","type":"print"},{"value":"2057-4347","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,3]]},"assertion":[{"value":"2020-06-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}