{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T04:45:49Z","timestamp":1774673149996,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T00:00:00Z","timestamp":1610582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007619","name":"Osteology Foundation","doi-asserted-by":"publisher","award":["16\u2010142"],"award-info":[{"award-number":["16\u2010142"]}],"id":[{"id":"10.13039\/501100007619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp; Exp Dental Res"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Platelet\u2010rich fibrin (PRF), an autogenous blood concentrate, contains multiple growth factors and is used as an adjunct in the periodontal regeneration and implant site development procedures to stimulate wound healing. Patient\u2010related factors such as chronic periodontitis may affect the quality of PRF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aimed to investigate and compare PRF's effects from patients diagnosed with generalized moderate or severe chronic periodontitis to patients who presented with intact periodontium on human gingival fibroblast (HGF) and human periodontal ligament fibroblast (HPLF) proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>A total of 33\u2009ml of whole intravenous blood was collected from each subject and centrifuged at 2700\u2009rpm for 12\u2009min in three 10\u00a0ml tubes, and 3\u00a0ml of blood was used for Complete Blood Count analysis. Three PRF clots were compressed to produce the membranes and liquid exudate. PRF membrane and 10% liquid exudate were exposed to 20,000 HPLFs\/well or 25,000 HGFs\/well in triplets from each subject in a 48 cell well plate. After 72\u2009h of incubation, the conditioned media were evaluated by Water Soluble Tetrazolium\u20101 assays to determine fibroblast proliferation. Controls included cells alone and media without cells. Complete blood counts were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Subjects in both groups were age and gender\u2010matched (intact 46.7\u2009\u00b1\u200911.4\u2009years and periodontitis 54.8\u2009\u00b1\u200910.4\u2009years, <jats:italic>p<\/jats:italic>\u2010value\u00a0=\u00a00.1344). Body Mass Index and White Blood Corpuscles in the periodontitis group was significantly higher than the intact group (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0176 and <jats:italic>p<\/jats:italic>\u00a0=\u00a00.0038) whereas no differences were seen for Red Blood Corpuscles (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.2020), Hemoglobin (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.2290) and Platelets (<jats:italic>p<\/jats:italic>\u00a0=\u00a04,094). There were no significant differences in the HGF and HPLF proliferation with PRF exudates and membranes between intact periodontium and periodontitis groups (all <jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). However, PRF exudates in both groups induced significant more cell proliferation when compared to PRF membranes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PRF exudates induced significant proliferation of fibroblasts and can play a vital role in wound healing. The current study concluded that PRF membranes, in combination with PRF exudates, can be utilized for their therapeutic and wound healing potential, not affected by the periodontal condition of the patient.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.370","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T19:26:50Z","timestamp":1610738810000},"page":"436-442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of platelet\u2010rich fibrin on human gingival and periodontal ligament fibroblast proliferation from chronic periodontitis versus periodontally healthy subjects"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2782-1947","authenticated-orcid":false,"given":"Apoorv","family":"Goel","sequence":"first","affiliation":[{"name":"Department of Periodontology Meharry Medical College School of Dentistry  Nashville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Jack","family":"Windsor","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Comprehensive Care Indiana University School of Dentistry  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard L.","family":"Gregory","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Comprehensive Care Indiana University School of Dentistry  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven B.","family":"Blanchard","sequence":"additional","affiliation":[{"name":"Department of Periodontology Indiana University School of Dentistry  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Hamada","sequence":"additional","affiliation":[{"name":"Department of Periodontology Indiana University School of Dentistry  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sdentj.2011.10.006"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01100.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/19.6.615"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-019-02944-7"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2005.07.012"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920112800624391"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2005.07.008"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.090531"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2016.160443"},{"issue":"7","key":"e_1_2_7_11_1","first-page":"1300","article-title":"How obesity affects the neutrophil\/lymphocyte and platelet\/lymphocyte ratio, systemic immune\u2010inflammatory index and platelet indices: A retrospective study","volume":"20","author":"Furuncuo\u011flu Y.","year":"2016","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-468694"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2010.01331.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.120388"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-016-1719-1"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4317\/jced.51292"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920112800624472"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/957518"},{"issue":"7","key":"e_1_2_7_19_1","first-page":"504","article-title":"Effects of plasma rich in growth factors and platelet\u2010rich fibrin on proliferation and viability of human gingival fibroblasts","volume":"12","author":"Vahabi S.","year":"2015","journal-title":"Journal of Dentistry (Tehran)"},{"issue":"5","key":"e_1_2_7_20_1","first-page":"694","article-title":"Local and systemic immune responses in gingivitis and periodontitis","volume":"9","author":"Zekonis G.","year":"2014","journal-title":"Central European Journal of Medicine"}],"container-title":["Clinical and Experimental Dental 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