{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T22:43:44Z","timestamp":1762037024704,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T00:00:00Z","timestamp":1620259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp; Exp Dental Res"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The use of adjunct post\u2010treatment mouth rinses containing chlorhexidine (CHX) for periodontitis patients is associated with side effects that reduce patient compliance. Our aim was to evaluate the proinflammatory and cell proliferation effects of an activated\u2010zinc mouth rinse (SM) that has been suggested as an alternative post\u2010treatment therapeutic.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Tissue models of gingival epithelium were used to simulate periodontal disease and compare inflammatory reactions after treatment with CHX or SM. Tissues were exposed to <jats:italic>Porphyromonas gingivalis<\/jats:italic> LPS and wounded to simulate periodontal disease. Tissues were treated and incubated for 6, 12, or 24\u2009h. Inflammatory cytokines were measured in culture medium by ELISA and local expression of Toll\u2010like receptor (TLR)\u20104 and proliferation marker Ki\u201067 was visualized by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SM and CHX treatments decreased secretion of IL\u20101\u03b2 and IL\u20108 into culture media at all time points. IL\u20101\u03b2 secretion levels were further decreased by SM compared to CHX treatment at all time points. TLR\u20104 expression appeared significantly increased 12\u2009h post\u2010treatment in the CHX tissues but remained relatively low in SM tissues at all time points. Ki\u201067 results suggest that cell proliferation was increased in the SM tissues earlier than CHX tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data suggest that SM may reduce inflammation in gingival tissues.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.437","type":"journal-article","created":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T15:04:25Z","timestamp":1620486265000},"page":"995-1001","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["An <scp>activated\u2010zinc<\/scp> oral rinse reduces <scp>pro\u2010inflammatory<\/scp> cytokine secretion and promotes proliferation in <i>Porphyromonas gingivalis<\/i><scp>LPS\u2010challenged<\/scp> gingival tissues \u2013 A pilot study"],"prefix":"10.1002","volume":"7","author":[{"given":"Kaitlyn A.","family":"Stanton","sequence":"first","affiliation":[{"name":"Southern Illinois University School of Dental Medicine  Alton Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9122-2551","authenticated-orcid":false,"given":"Barbara A.","family":"McCracken","sequence":"additional","affiliation":[{"name":"Southern Illinois University School of Dental Medicine  Alton Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2007.01103.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13046"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-019-0068-8"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.adaj.2017.01.021"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.11.4569-4574.1993"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512457373"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000006932"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0901-5027(88)80222-4"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2007.00335.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12194"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0089-x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1986.tb01517.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10010016"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.51107"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/130.5.1500S"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.05.012"},{"issue":"4","key":"e_1_2_10_18_1","first-page":"339","article-title":"Comparative evaluation of SmartMouth clinical DDS advanced oral rinse and chlorhexidine mouthrinse","volume":"17","author":"Miley D. 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