{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:05:54Z","timestamp":1764687954622,"version":"3.40.5"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T00:00:00Z","timestamp":1635811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp; Exp Dental Res"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Besides angiotensin converting enzyme 2 (ACE2), an active involvement of proteases (FURIN and\/or TMPRSS2) is important for cellular entry of SARS\u2010CoV\u20102. Therefore, a simultaneous expression profiling of entry proteins in a tissue might provide a better risk assessment of SARS\u2010CoV\u20102 infection as compared to individual proteins. In an attempt to understand the relative susceptibility of oral squamous cell carcinoma (OSCC) lesions as compared to the normal oral mucosa (NOM) for SARS\u2010CoV\u20102 attachment\/entry, this study examined the mRNA and protein expression profiles of ACE2, FURIN, and TMPRSS2 in the corresponding tissues using public transcriptomic and proteomics datasets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and methods<\/jats:title><jats:p>Public transcriptomic and proteomics datasets (the Cancer Genome Atlas (TCGA)\/the Genotype\u2010Tissue Expression (GTEx), the Human Protein Atlas (HPA), and two independent microarray datasets) were used to examine the expression profiles of ACE2, TMPRSS2 and FURIN in NOM and OSCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>ACE2<\/jats:italic>, <jats:italic>TMPRSS2<\/jats:italic>, and <jats:italic>FURIN<\/jats:italic> mRNAs were detected in NOM, however, at lower levels as compared to other body tissues. Except for moderate up\u2010regulation of <jats:italic>FURIN<\/jats:italic>, expression levels of <jats:italic>ACE2<\/jats:italic> and <jats:italic>TMPRSS2<\/jats:italic> mRNA were unchanged\/down\u2010regulated in OSCC as compared to the NOM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate that NOM may serve as a possible site for SARS\u2010CoV\u20102 attachment, however, to a lesser extent as compared to organs with higher expression levels of the SARS\u2010CoV\u20102 entry proteins. However, the evidence is lacking to suggest that expression status of entry proteins predisposes OSCC lesions to additional risk for SARS\u2010CoV\u20102 attachment\/entry as compared to NOM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.510","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T09:40:21Z","timestamp":1635846021000},"page":"117-122","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Expression profile of <scp>SARS\u2010CoV\u20102<\/scp> cellular entry proteins in normal oral mucosa and oral squamous cell carcinoma"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0061-825X","authenticated-orcid":false,"given":"Dipak","family":"Sapkota","sequence":"first","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunita","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1687-3247","authenticated-orcid":false,"given":"Tine M.","family":"S\u00f8land","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"},{"name":"Department of Pathology Rikshospitalet, Oslo University Hospital  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1842-9521","authenticated-orcid":false,"given":"Paulo H.","family":"Braz\u2010Silva","sequence":"additional","affiliation":[{"name":"Department of Stomatology, School of Dentistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Laboratory of Virology, Institute of Tropical Medicine of S\u00e3o Paulo, School of Medicine University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muy\u2010Teck","family":"Teh","sequence":"additional","affiliation":[{"name":"Centre for Oral Immunobiology and Regenerative Medicine, Institute of Dentistry, Barts and The London School of Medicine and Dentistry Queen Mary University of London  London UK"},{"name":"China\u2010British Joint Molecular Head and Neck Cancer Research Laboratory Affiliated Stomatological Hospital of Guizhou Medical University  Guiyang 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