{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T16:16:41Z","timestamp":1779380201070,"version":"3.53.1"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:00:00Z","timestamp":1663113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp; Exp Dental Res"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To evaluate intermediate treatments between sodium hypochlorite and chlorhexidine gluconate irrigations for the prevention of a toxic brown precipitate in root canal therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Thirty\u2010nine premolars were irrigated with 6% sodium hypochlorite and divided into either: No intermediate treatment; Dry paper points; three different irrigations with 17% ethylenediaminetetraacetic acid, deionized water, or 5% sodium thiosulfate. 2% chlorhexidine gluconate was the final irrigant in all groups. Sectioned teeth were analyzed for brown precipitate intensity and area using stereomicroscopy and components related to para\u2010chloroaniline using Time\u2010of\u2010Flight Secondary Ion Mass Spectrometry (ToF\u2010SIMS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Stereomicroscopy showed that 5% STS significantly reduced brown precipitate intensity and area as compared with no intermediate irrigation (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05, Chi\u2010square, generalized linear model, and Tukey's multiple comparison tests). Utilizing ToF\u2010SIMS, 5% sodium thiosulfate was most effective in reducing the components representing para\u2010chloroaniline and chlorhexidine gluconate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The 5% sodium thiosulfate was most effective among other intermediate treatments, assessed by stereomicroscopy and ToF\u2010SIMS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.654","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T09:39:57Z","timestamp":1663148397000},"page":"1591-1597","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate"],"prefix":"10.1002","volume":"8","author":[{"given":"Vashti","family":"Bueso","sequence":"first","affiliation":[{"name":"School of Dentistry The University of Texas Health Science Center at Houston Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Parikh","sequence":"additional","affiliation":[{"name":"Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center at Houston School of Dentistry Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanguy","family":"Terlier","sequence":"additional","affiliation":[{"name":"SIMS Laboratory, Shared Equipment Authority Rice University Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian N.","family":"Holland","sequence":"additional","affiliation":[{"name":"Office of Research, The University of Texas Health Science Center at Houston School of Dentistry Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nima D.","family":"Sarmast","sequence":"additional","affiliation":[{"name":"Department of Endodontics, The University of Texas Health Science Center at Houston School of Dentistry Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1456-0730","authenticated-orcid":false,"given":"Ji Wook","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Endodontics, The University of Texas Health Science Center at Houston School of Dentistry Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/aej.12562"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.10.031"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.04.001"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.11.006"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/JCD.JCD_72_18"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(91)90166-5"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-013-1049-5"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2003.00670.x"},{"key":"e_1_2_11_10_1","first-page":"390","article-title":"Quantity of sodium thiosulfate required to neutralize various concentrations of sodium hypochlorite","volume":"2","author":"Hegde J.","year":"2012","journal-title":"Asian Journal of Pharmaceutical Sciences"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13583"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2010.01.012"},{"key":"e_1_2_11_13_1","first-page":"1","article-title":"Microbiological examination of root canals and periapical tissues of human teeth. 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