{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T02:27:33Z","timestamp":1788488853291,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T00:00:00Z","timestamp":1499040000000},"content-version":"vor","delay-in-days":32,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005293","name":"G\u00f6teborgs Tandl\u00e4kare-S\u00e4llskap","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005293","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Exp Dental Res"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim was to investigate if hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) induces the formation of the NLRP3 inflammasome and subsequent IL\u20101\u03b2 and IL\u201018 secretion in human peripheral blood mononuclear cells (PBMCs) and in the human monocyte cell line THP1. Bacterial production of H<jats:sub>2<\/jats:sub>S has been suggested to participate in the inflammatory host response in periodontitis pathogenesis. H<jats:sub>2<\/jats:sub>S is a toxic gas with pro\u2010inflammatory properties. It is produced by bacterial degradation of sulfur\u2010containing amino acids, for example, cysteine. We hypothesize that H<jats:sub>2<\/jats:sub>S affects the inflammatory host response by inducing formation of the NLRP3 inflammasome and thereby causes the secretion of IL\u20101\u00df and IL\u201018. PBMCs from eight healthy blood donors, the human monocyte cell line THP1 Null, and two variants of the THP1 cell line unable to form the NLRP3 inflammasome were cultured in the presence or absence of 1\u00a0mM sodium hydrosulfide (NaHS) in 24\u2010well plates at 37\u00b0C for 24\u00a0hr. Supernatants were collected and the IL\u20101\u03b2 and IL\u201018 concentrations were measured with DuoSet ELISA Development kit. PBMCs exposed to NaHS produced more IL\u20101\u00df and IL\u201018 than unexposed control cells (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.023 and <jats:italic>p<\/jats:italic>\u00a0=\u00a0.008, respectively). An increase of extracellular potassium ions (K<jats:sup>+<\/jats:sup>) inhibited the secretion of IL\u20101\u00df and IL\u201018 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.008). Further, NaHS triggered the secretion of IL\u20101\u00df and IL\u201018 in human THP1\u2010Null monocytes (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0006 and <jats:italic>p<\/jats:italic>\u00a0=\u00a0.002, respectively), while the NaHS\u2010dependent secretion was reduced in the monocyte cell lines unable to form the NLRP3 inflammasome. Hence, the results suggest that NaHS induces the formation of the NLRP3 inflammasome and thus the secretion of IL\u20101\u00df and IL\u201018. Enhanced NLRP3 inflammasome\u2010dependent secretion of IL\u20101\u03b2 and IL\u201018 in human mononuclear leukocytes exposed to NaHS <jats:italic>in vitro<\/jats:italic> is reported. This may be a mode for H<jats:sub>2<\/jats:sub>S to contribute to the inflammatory host response and pathogenesis of periodontal disease.<\/jats:p>","DOI":"10.1002\/cre2.69","type":"journal-article","created":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T09:25:06Z","timestamp":1499073906000},"page":"115-120","source":"Crossref","is-referenced-by-count":28,"title":["Hydrogen sulfide exposure induces NLRP3 inflammasome\u2010dependent IL\u20101\u03b2 and IL\u201018 secretion in human mononuclear leukocytes <i>in vitro<\/i>"],"prefix":"10.1002","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2217-3642","authenticated-orcid":false,"given":"Amina","family":"Basic","sequence":"first","affiliation":[{"name":"Department of Oral Microbiology and Immunology Institute of Odontology, Sahlgrenska Academy, University of Gothenburg  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