{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:45:17Z","timestamp":1788576317798,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T00:00:00Z","timestamp":1502323200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Exp Dental Res"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The surface pre\u2010reacted glass ionomer (S\u2010PRG) filler, a component of composite resin, is capable of releasing metal ions that possess antibacterial activity against caries and periodontal pathogens. Although S\u2010PRG has been suggested to be involved in oral disease prevention, no reports have been published regarding its preventive effect on periodontal disease <jats:italic>in vivo<\/jats:italic>. The present study investigated whether the eluate from S\u2010PRG (S\u2010PRG eluate) has a suppressive effect on tissue destruction induced in a mouse model of ligature\u2010induced periodontal disease. Twenty\u2010seven C57BL\/6 mice were divided into three groups of nine animals each, no ligature group (Lig(\u2212)), ligature group (Lig(+)S\u2010PRG(\u2212)) and ligature with S\u2010PRG eluate group (Lig(+)S\u2010PRG(+)). Alveolar bone loss was evaluated using micro\u2010computed tomography scanning. Histologic changes were detected by hematoxylin and eosin staining. The infiltration of inflammatory cells was assessed by Ly6G and F4\/80 staining immunohistochemically. The distribution of metal ions was detected by time\u2010of\u2010flight secondary ion mass spectrometry. S\u2010PRG eluate clearly inhibited alveolar bone loss and bone density. The histological analysis revealed that S\u2010PRG eluate reduced destruction of the collagen bundle in the periodontal ligament and the infiltration of inflammatory cells. Immunohistochemical analysis showed that the S\u2010PRG eluate significantly suppressed the number of infiltrating neutrophils and macrophages. Time\u2010of\u2010flight secondary ion mass spectrometry analysis revealed that more boron ions were present in the Lig(+)S\u2010PRG(+) group than in the Lig(+)S\u2010PRG(\u2212) group. Our results suggest that the S\u2010PRG eluate has a preventive effect against tissue destruction in periodontal disease through its anti\u2010inflammatory effects <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/cre2.70","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T03:14:38Z","timestamp":1502421278000},"page":"126-133","source":"Crossref","is-referenced-by-count":17,"title":["Metal ions from S\u2010PRG filler have the potential to prevent periodontal disease"],"prefix":"10.1002","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9770-8109","authenticated-orcid":false,"given":"Yoko","family":"Iwamatsu\u2010Kobayashi","sequence":"first","affiliation":[{"name":"Division of Operative Dentistry, Department of Restorative Dentistry Tohoku University Graduate School of Dentistry  Sendai Miyagi 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