{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T14:46:56Z","timestamp":1778770016702,"version":"3.51.4"},"reference-count":61,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,11,3]],"date-time":"2024-11-03T00:00:00Z","timestamp":1730592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p><jats:italic>Fusobacterium<\/jats:italic> can contribute to oral diseases, but also pose as a systemic risk factor. This genus, and especially <jats:italic>F. nucleatum<\/jats:italic>, can be found in colorectal cancer (CRC) tissue and is involved in multiple aspects of this type of cancer. Previous studies indicated a possible oral origin of these bacteria; however, stronger evidence is needed to reach a definitive conclusion. This pilot study aimed to establish a method to successfully compare, at the strain level, fusobacteria from the oral cavity and CRC resection material for future cohort studies of CRC patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>In a first cohort of eight periodontitis patients, gingival crevicular fluid and saliva were collected. <jats:italic>Fusobacterium<\/jats:italic> was isolated on two different media. In a second cohort, saliva and CRC resection material were collected from ten CRC patients. These samples were used for screening of <jats:italic>Fusobacterium<\/jats:italic> with culturing, 16S rRNA gene profiling and a PCR\u2010based approach.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the first cohort, different <jats:italic>Fusobacterium<\/jats:italic> species were identified in GCF and saliva samples. However, as the total yield of <jats:italic>Fusobacterium<\/jats:italic> seemed slightly higher in saliva samples, it was therefore preferred for subsequent sample collection. Thus, in the second cohort, patient\u2010matched saliva and CRC resection material were screened for <jats:italic>Fusobacterium<\/jats:italic> and this showed that nine patients were culture\u2010positive in the saliva samples; however, no <jats:italic>Fusobacterium<\/jats:italic> could be isolated from the resection material. On the other hand, 16S rRNA gene profiling of the resection material indicated that eight CRC patients were positive for <jats:italic>Fusobacterium<\/jats:italic>. All eight of these patients carried <jats:italic>Fusobacterium<\/jats:italic> in their saliva, indicated by both marker gene PCR and culture\u2010based screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These pilot results are compatible with data from previous studies, indicating a possible link between oral and CRC\u2010associated <jats:italic>Fusobacterium<\/jats:italic>, and a more in\u2010depth analysis of specific strains and their characteristics in a larger cohort is justified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Trial Registration<\/jats:title><jats:p>The protocol was registered at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/clinicaltrials.gov\">clinicaltrials.gov<\/jats:ext-link> (NCT05945082).<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.70016","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T06:30:23Z","timestamp":1730701823000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Does <i>Fusobacterium<\/i> in Colorectal Cancer Sites Originate From the Oral Cavity? A Pilot Study"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1434-5010","authenticated-orcid":false,"given":"Niels","family":"Plomp","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen University of Groningen Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8279-7943","authenticated-orcid":false,"given":"Kristina","family":"Bertl","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Dental Clinic, Faculty of Medicine Sigmund Freud University Vienna Vienna Austria"},{"name":"Department of Periodontology Blekinge Hospital Karlskrona Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4803-8548","authenticated-orcid":false,"given":"Marie\u2010Louise","family":"Lydrup","sequence":"additional","affiliation":[{"name":"Department of Surgery Sk\u00e5ne University Hospital and Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8700-9141","authenticated-orcid":false,"given":"Klas","family":"Sj\u00f6berg","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Lund University Malm\u00f6 Sweden"},{"name":"Department of Gastroenterology and Nutrition Sk\u00e5ne University Hospital Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2725-6148","authenticated-orcid":false,"given":"Hermie J. 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