{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:01:59Z","timestamp":1792623719724,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Peri\u2010implant diseases (PID), specifically peri\u2010implant mucositis and peri\u2010implantitis, significantly jeopardize dental implant success. Traditional diagnostic methods, relying on periodontal probing and radiographs, have limitations in precisely diagnosing these conditions. Recent studies suggest that microRNA (miRNA) profiling might be a breakthrough in the early detection of PID. This study aimed to evaluate the expression levels of specific miRNAs\u2014miR\u201098, miR\u2010145, miR\u2010146a, and let\u20107e\u2014in individuals with peri\u2010implant mucositis and peri\u2010implantitis compared to a healthy control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>From January 2022 to October 2023, 19 participants with 38 dental implants were recruited according to established criteria. After clinical and radiographic evaluations, dental implants were grouped as peri\u2010implant health (Group 1), peri\u2010implant mucositis (Group 2), and peri\u2010implantitis (Group 3). Subsequently, peri\u2010implant crevicular fluid (PICF) samples were collected and analyzed through real\u2010time quantitative polymerase chain reaction (qPCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Based on the findings, only let\u20107e biomarkers were consistently detected across all samples, with their expression increased eightfold in peri\u2010implant mucositis and 94\u2010fold in peri\u2010implantitis cases. Kruskal\u2013Wallis's test showed a statistically significant difference in relative gene expression of let\u20107e between the different groups, <jats:italic>H<\/jats:italic>(2)\u2009=\u200925.825, <jats:italic>p<\/jats:italic>\u2009\u2264\u20090.001.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study indicates that miRNA biomarkers, particularly let\u20107e, may play a significant role in the development and progression of PID, highlighting the need for further in\u2010depth investigation, with PICF offering a practical, noninvasive sampling method.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.70044","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T16:41:16Z","timestamp":1746463276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Let\u20107e as Potential Diagnostic Biomarkers in Early Detection of Peri\u2010Implant Diseases: A Cross\u2010Sectional Study"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4320-3284","authenticated-orcid":false,"given":"Nurul Latifah","family":"Zainal Abidin","sequence":"first","affiliation":[{"name":"Centre of Periodontology Studies University Technology MARA Sungai Buloh Campus, Jalan Hospital Sungai Buloh Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norjehan","family":"Latib","sequence":"additional","affiliation":[{"name":"Periodontic Unit Kota Damansara Dental Clinic Petaling Jaya Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fouad Hussain","family":"Al\u2010Bayaty","sequence":"additional","affiliation":[{"name":"Department of Postgraduate Study Malaysia MAHSA University Jenjarom Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Faizal Hafez","family":"Hidayat","sequence":"additional","affiliation":[{"name":"Centre of Periodontology Studies University Technology MARA Sungai Buloh Campus, Jalan Hospital Sungai Buloh Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1989.tb00863.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12957"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4317\/jced.51106"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2012.09.015"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.20-0372"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.4324\/9780203771587"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24061034"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201242702"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2008.01275.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2019.03.008"},{"issue":"3","key":"e_1_2_10_12_1","first-page":"109","article-title":"Periodontopathogen LPSs Regulate MicroRNA Expression in Human Gingival Epithelial Cells","volume":"36","author":"Lee H. 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