{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:55:14Z","timestamp":1790380514362,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008376","name":"Helsingin ja Uudenmaan Sairaanhoitopiiri","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The objective of our study was to re\u2010evaluate periapical lesions, including radicular cysts (RCs) and periapical granulomas (PGs) for locations, histopathological features, and degree of fibrosis in relation to the inflammatory response. In addition, we examined the presence of\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      (Pg) and\n                      <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                      (Fn) since both are widely recognized pathogens in periodontal infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We re\u2010evaluated samples of RCs and PGs (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009728) and collected data for analyses by IBM's SPSS Statistics. Among these samples, we stained 93 samples to determine the immunoexpression of Pg and Fn. For immunostaining, we used Gingipain R1 antibody for Pg and Rabbit anti\u2010Fn antibody for Fn.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Fibrosis is associated with mild inflammation. We found a significant positive correlation between Pg and Fn. Thus, these pathogens are likely to occur together in periapical inflammatory lesions. We additionally noted that these periodontopathic pathogens are more likely to be present in RCs than in PGs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Asymptomatic radiologically diagnosed periapical lesions may not necessarily need root canal retreatment in healthy patients since these lesions may represent scar tissue rather than active apical periodontitis. Clinical and radiological follow\u2010up is still needed. Yet, periapical lesions, especially cysts, may contain dystopic periodontopathic pathogens, and Pg and Fn often occur together in periapical lesions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cre2.70098","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:39:31Z","timestamp":1739925571000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Radicular Cysts and Periapical Granulomas: Data Documentation for 696 Cases and Findings on Fibrosis, and\n                    <i>Porphyromonas gingivalis<\/i>\n                    and\n                    <i>Fusobacterium nucleatum<\/i>\n                    in These Lesions"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5376-4846","authenticated-orcid":false,"given":"Sirke","family":"Virkkunen","sequence":"first","affiliation":[{"name":"Department of Pathology University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terhi","family":"Kaarela","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merja","family":"Laine","sequence":"additional","affiliation":[{"name":"Department of Dentistry University of Turku Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auli","family":"Suominen","sequence":"additional","affiliation":[{"name":"Department of Dentistry University of Turku Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaana","family":"Hagstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Helsinki and Helsinki University Hospital Helsinki Finland"},{"name":"Department of Oral Pathology and Radiology University of Turku Turku Finland"},{"name":"Translational Cancer Medicine Research Program, Faculty of Medicine University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases University of Helsinki and Helsinki University Hospital Helsinki Finland"},{"name":"Section of Periodontology and Dental Prevention, Department of Dental Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caj","family":"Haglund","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Helsinki and Helsinki University Hospital Helsinki Finland"},{"name":"Translational Cancer Medicine Research Program, Faculty of Medicine University of Helsinki Helsinki Finland"},{"name":"Department of Surgery University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-020-03510-2"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.9.1.55"},{"issue":"6","key":"e_1_2_10_4_1","first-page":"311","article-title":"A Study of Odontogenic Cysts With Special Reference to Comparisons Between Keratinizing and Non\u2010Keratinizing Cysts","volume":"67","author":"Borg G.","year":"1974","journal-title":"Svensk tandlakare tidskrift. 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