{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T23:53:27Z","timestamp":1776297207700,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,23]],"date-time":"2025-02-23T00:00:00Z","timestamp":1740268800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004257","name":"Helse Vest","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004257","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Cell secretomes represent a promising strategy for periodontal and bone regeneration. The objective of this study was to characterize the secretome of human gingival fibroblasts (GF) from periodontally diseased tissues (GF\u2010perio) using proteomics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Conditioned media of GF\u2010perio from periodontitis patients (<jats:italic>n<\/jats:italic>\u2009=\u20096, 48\u2010h serum\u2010free culture) were subjected to liquid chromatography with tandem mass spectrometry. Global profiles, differentially expressed proteins (DEPs), and functional\/gene\u2010set enrichment (FEA) were analyzed using bioinformatics. Selected bone regeneration\u2010related proteins were additionally measured using a multiplex immunoassay. Conditioned media of GF from periodontally healthy subjects were used as a reference.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 1833 proteins were detected in GF\u2010perio secretomes, including several growth factors, cytokines, chemokines, and extracellular matrix proteins important for wound healing and regeneration. Key bone\u2010related cytokines (FGF2, MCP1, GPNMB, MMP2, IL6, IL8) were confirmed by an immunoassay. Compared to the reference group, 127 exclusive proteins and 73 DEPs (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) were identified in the GF\u2010perio group. FEA revealed significant enrichment of \u201cexosome\u201d and \u201ccytoplasm\u201d related cellular components in GF\u2010perio secretomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The secretome of GF from periodontally diseased tissues may hold therapeutic potential, with several proteins important for wound healing and regeneration, especially those related to exosome functions.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.70103","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:31:17Z","timestamp":1741743077000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Secretomes of Gingival Fibroblasts From Periodontally Diseased Tissues: A Proteomic Analysis"],"prefix":"10.1002","volume":"11","author":[{"given":"Anne Kari","family":"Smed\u00e5s","sequence":"first","affiliation":[{"name":"Department of Clinical Dentistry, Faculty of Medicine 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