{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T12:13:47Z","timestamp":1782303227732,"version":"3.54.5"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to verify the methodology for investigation of interleukin (IL) concentrations in peri\u2010implant crevicular fluid (PICF) and to assess their correlation with clinical parameters, particularly bleeding on probing (BOP) and probing pocket depth (PPD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>PICF was collected from 60 peri\u2010implantitis patients and 25 healthy volunteers to analyze IL levels in both groups. Sampling was conducted using sterile dental brushes, which were placed in the peri\u2010implant crevice for up to 10\u2009s. The brushes were then stored in Eppendorf tubes containing 1\u2009mL of phosphate\u2010buffered saline and centrifuged at 3000\u2009rpm for 15\u2009min. Samples were frozen at \u221280\u00b0C (193,15\u2009K) for preservation. IL concentrations were measured using enzyme\u2010linked immunosorbent assays (ELISA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The study found significantly elevated levels of IL\u20101\u03b1, IL\u20106, IL\u20104, and IL\u201010 in PI patients compared to healthy controls. Correlation analysis revealed strong associations between BOP and IL\u20101\u03b1, IL\u20106, and IL\u201010, while PPD showed no significant relationship with IL levels. Among these, IL\u201010 had the greatest influence on BOP, highlighting its dual role in reducing excessive inflammation and sustaining chronic immune responses through immune tolerance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The present study demonstrated that IL\u20101\u03b1, IL\u20106, and IL\u201010 levels in PICF are closely associated with the severity of peri\u2010implant inflammation. Among these cytokines, IL\u201010 showed the strongest relationship with BOP, indicating its important role in the modulation of local immune activity. IL\u20106 was also positively correlated with inflammatory severity, reflecting its involvement in sustaining the destructive processes within peri\u2010implant tissues. Overall, the obtained data suggest that IL\u20106 and IL\u201010 may serve as useful indicators of ongoing inflammatory activity in peri\u2010implantitis. However, their prognostic or diagnostic utility requires confirmation in longitudinal studies assessing temporal cytokine changes and treatment outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cre2.70252","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T07:13:07Z","timestamp":1764659587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigation of the Impact of Peri\u2010Implantitis Processes on Interleukin Profiles in Peri\u2010Implant Crevicular Fluid"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6554-3814","authenticated-orcid":false,"given":"Kristina","family":"Volchykhina","sequence":"first","affiliation":[{"name":"Department of Dentistry of Postgraduate Education Zaporizhzhia State Medical and Pharmaceutical University Zaporizhzhia Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olesya","family":"Manukhina","sequence":"additional","affiliation":[{"name":"Department of Dentistry of Postgraduate Education Zaporizhzhia State Medical and Pharmaceutical University Zaporizhzhia Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9957-4247","authenticated-orcid":false,"given":"Ika Dewi","family":"Ana","sequence":"additional","affiliation":[{"name":"Dental Biomedical Sciences Department, Faculty of Dentistry Universitas Gadjah Mada Yogyakarta Indonesia"},{"name":"Research Collaboration Center for Biomedical Scaffolds National Research and Innovation Agency (BRIN) \u2013 Universitas Gadjah Mada (UGM) Yogyakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Beshchasna","sequence":"additional","affiliation":[{"name":"Dental Biomedical Sciences Department, Faculty of Dentistry Universitas Gadjah Mada 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